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The dumbbell shaped binuclear ruthenium complex DeltaDelta-P requires transiently melted DNA in order to thread through the DNA bases and intercalate DNA. Because such fluctuations are rare at room temperature, the binding rates are extremely low in bulk experiments. Here, single DNA molecule stretching is used to lower the barrier to DNA melting, resulting in direct mechanical manipulation of the barrier to DNA binding by the ligand. The rate of DNA threading depends exponentially on force, consistent with theoretical predictions. From the observed force dependence of the binding rate, we demonstrate that only one base pair must be transiently melted for DNA threading to occur.
Thayaparan Paramanathan, and
Fredrik Westerlund, and
Micah J McCauley, and
Ioulia Rouzina, and
Per Lincoln, and
Mark C Williams
February 2009,
Biochemistry,
Thayaparan Paramanathan, and
Fredrik Westerlund, and
Micah J McCauley, and
Ioulia Rouzina, and
Per Lincoln, and
Mark C Williams
March 2016,
Biophysical journal,
Thayaparan Paramanathan, and
Fredrik Westerlund, and
Micah J McCauley, and
Ioulia Rouzina, and
Per Lincoln, and
Mark C Williams
November 2008,
Journal of the American Chemical Society,
Thayaparan Paramanathan, and
Fredrik Westerlund, and
Micah J McCauley, and
Ioulia Rouzina, and
Per Lincoln, and
Mark C Williams
December 2011,
The journal of physical chemistry. B,
Thayaparan Paramanathan, and
Fredrik Westerlund, and
Micah J McCauley, and
Ioulia Rouzina, and
Per Lincoln, and
Mark C Williams
June 2001,
Journal of the American Chemical Society,
Thayaparan Paramanathan, and
Fredrik Westerlund, and
Micah J McCauley, and
Ioulia Rouzina, and
Per Lincoln, and
Mark C Williams
September 2010,
Chemistry (Weinheim an der Bergstrasse, Germany),
Thayaparan Paramanathan, and
Fredrik Westerlund, and
Micah J McCauley, and
Ioulia Rouzina, and
Per Lincoln, and
Mark C Williams
January 2021,
Physical chemistry chemical physics : PCCP,
Thayaparan Paramanathan, and
Fredrik Westerlund, and
Micah J McCauley, and
Ioulia Rouzina, and
Per Lincoln, and
Mark C Williams
February 1991,
Biochemistry,
Thayaparan Paramanathan, and
Fredrik Westerlund, and
Micah J McCauley, and
Ioulia Rouzina, and
Per Lincoln, and
Mark C Williams
November 2013,
Nano letters,
Thayaparan Paramanathan, and
Fredrik Westerlund, and
Micah J McCauley, and
Ioulia Rouzina, and
Per Lincoln, and
Mark C Williams
March 2019,
Journal of the American Chemical Society,
