Two postprocessing techniques for the elimination of background autofluorescence for fluorescence lifetime imaging microscopy. 2008

Phillip B Jones, and Aneta Rozkalne, and Melanie Meyer-Luehmann, and Tara L Spires-Jones, and Alexandra Makarova, and Anand T N Kumar, and Oksana Berezovska, and Brian B Bacskai, and Bradley T Hyman
Harvard Medical School, Massachusetts General Hospital, MassGeneral Institute for Neurodegenerative Disorders, 114 16th Street, Charlestown, Massachusetts 02129, USA.

The analysis of fluorescence lifetime imaging microscopy (FLIM) data under complex biological conditions can be challenging. Particularly, the presence of short-lived autofluorescent aggregates can confound lifetime measurements in fluorescence energy transfer (FRET) experiments, where it can become confused with the signal from exogenous fluorophores. Here we report two techniques that can be used to discriminate the contribution of autofluorescence from exogenous fluorphores in FLIM. We apply the techniques to transgenic mice that natively express yellow fluorescence protein (YFP) in a subset of cortical neurons and to histological slices of aged human brain tissue, where we study the misfolding of intracellular tau protein in the form of neurofibrillary tangles.

UI MeSH Term Description Entries
D007089 Image Enhancement Improvement of the quality of a picture by various techniques, including computer processing, digital filtering, echocardiographic techniques, light and ultrastructural MICROSCOPY, fluorescence spectrometry and microscopy, scintigraphy, and in vitro image processing at the molecular level. Image Quality Enhancement,Enhancement, Image,Enhancement, Image Quality,Enhancements, Image,Enhancements, Image Quality,Image Enhancements,Image Quality Enhancements,Quality Enhancement, Image,Quality Enhancements, Image
D007090 Image Interpretation, Computer-Assisted Methods developed to aid in the interpretation of ultrasound, radiographic images, etc., for diagnosis of disease. Image Interpretation, Computer Assisted,Computer-Assisted Image Interpretation,Computer-Assisted Image Interpretations,Image Interpretations, Computer-Assisted,Interpretation, Computer-Assisted Image,Interpretations, Computer-Assisted Image
D008822 Mice, Transgenic Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN. Transgenic Mice,Founder Mice, Transgenic,Mouse, Founder, Transgenic,Mouse, Transgenic,Mice, Transgenic Founder,Transgenic Founder Mice,Transgenic Mouse
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D000465 Algorithms A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task. Algorithm
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013382 Subtraction Technique Combination or superimposition of two images for demonstrating differences between them (e.g., radiograph with contrast vs. one without, radionuclide images using different radionuclides, radiograph vs. radionuclide image) and in the preparation of audiovisual materials (e.g., offsetting identical images, coloring of vessels in angiograms). Subtraction Technic,Subtraction Technics,Subtraction Techniques,Technic, Subtraction,Technics, Subtraction,Technique, Subtraction,Techniques, Subtraction
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D016477 Artifacts Any visible result of a procedure which is caused by the procedure itself and not by the entity being analyzed. Common examples include histological structures introduced by tissue processing, radiographic images of structures that are not naturally present in living tissue, and products of chemical reactions that occur during analysis. Artefacts,Artefact,Artifact

Related Publications

Phillip B Jones, and Aneta Rozkalne, and Melanie Meyer-Luehmann, and Tara L Spires-Jones, and Alexandra Makarova, and Anand T N Kumar, and Oksana Berezovska, and Brian B Bacskai, and Bradley T Hyman
January 2003, Methods in cell biology,
Phillip B Jones, and Aneta Rozkalne, and Melanie Meyer-Luehmann, and Tara L Spires-Jones, and Alexandra Makarova, and Anand T N Kumar, and Oksana Berezovska, and Brian B Bacskai, and Bradley T Hyman
January 1998, Methods in cell biology,
Phillip B Jones, and Aneta Rozkalne, and Melanie Meyer-Luehmann, and Tara L Spires-Jones, and Alexandra Makarova, and Anand T N Kumar, and Oksana Berezovska, and Brian B Bacskai, and Bradley T Hyman
January 2006, Methods in enzymology,
Phillip B Jones, and Aneta Rozkalne, and Melanie Meyer-Luehmann, and Tara L Spires-Jones, and Alexandra Makarova, and Anand T N Kumar, and Oksana Berezovska, and Brian B Bacskai, and Bradley T Hyman
January 2021, Eye (London, England),
Phillip B Jones, and Aneta Rozkalne, and Melanie Meyer-Luehmann, and Tara L Spires-Jones, and Alexandra Makarova, and Anand T N Kumar, and Oksana Berezovska, and Brian B Bacskai, and Bradley T Hyman
January 2007, Methods in cell biology,
Phillip B Jones, and Aneta Rozkalne, and Melanie Meyer-Luehmann, and Tara L Spires-Jones, and Alexandra Makarova, and Anand T N Kumar, and Oksana Berezovska, and Brian B Bacskai, and Bradley T Hyman
December 2004, Current protocols in cell biology,
Phillip B Jones, and Aneta Rozkalne, and Melanie Meyer-Luehmann, and Tara L Spires-Jones, and Alexandra Makarova, and Anand T N Kumar, and Oksana Berezovska, and Brian B Bacskai, and Bradley T Hyman
January 2013, Methods in cell biology,
Phillip B Jones, and Aneta Rozkalne, and Melanie Meyer-Luehmann, and Tara L Spires-Jones, and Alexandra Makarova, and Anand T N Kumar, and Oksana Berezovska, and Brian B Bacskai, and Bradley T Hyman
January 2005, Advances in biochemical engineering/biotechnology,
Phillip B Jones, and Aneta Rozkalne, and Melanie Meyer-Luehmann, and Tara L Spires-Jones, and Alexandra Makarova, and Anand T N Kumar, and Oksana Berezovska, and Brian B Bacskai, and Bradley T Hyman
January 2004, Microscopy research and technique,
Phillip B Jones, and Aneta Rozkalne, and Melanie Meyer-Luehmann, and Tara L Spires-Jones, and Alexandra Makarova, and Anand T N Kumar, and Oksana Berezovska, and Brian B Bacskai, and Bradley T Hyman
January 2021, Advances in experimental medicine and biology,
Copied contents to your clipboard!