Biomaterial Database

Secretoglobins: lacrimal gland-specific rabbit lipophilin mRNAs. 2008

Susann G Remington, and Jean M Crow, and J Daniel Nelson

Department of Ophthalmology, HealthPartners Medical Group and Clinics, St. Paul, Minnesota, USA. susann.g.remington@healthpartners.com

OBJECTIVE RNAs encoding secretoglobin subunits, also known as lipophilins, are found in rabbit lacrimal glands. Some secretoglobins in other species are tissue- and/or sex-specific, whereas other secretoglobins are expressed in a variety of tissues of both sexes. In this study the tissue-specific and sexual dimorphic expression patterns of rabbit lacrimal gland lipophilins AL, AL2, BL, CL, and CL2 were determined. METHODS RNAs from male and female rabbit lacrimal glands were compared in a differential display analysis, and a new rabbit lacrimal gland secretoglobin, lipophilin AL2, was identified. Next, RNAs from male and female rabbit harderian, lacrimal, mandibular, sublingual, and parotid glands and from liver, kidney, pancreas, testis (male), ovary, and mammary gland (female) were isolated, electrophoresed in agarose gels, and transferred to nylon membranes. cDNA probes encoding the lipophilins AL, AL2, BL, and CL/CL2 were hybridized to the RNA in the blots. RESULTS Rabbit mRNAs encoding the lipophilins AL, AL2, BL, CL, and CL2 were detected only in the lacrimal gland. Lipophilin AL2 mRNA was detected only in male rabbit lacrimal gland. CONCLUSIONS In the rabbit, several lipophilins were expressed only in the lacrimal gland.

UI	MeSH Term	Description	Entries
D007765	Lacrimal Apparatus	The tear-forming and tear-conducting system which includes the lacrimal glands, eyelid margins, conjunctival sac, and the tear drainage system.	Lacrimal Gland,Nasolacrimal Apparatus,Conjunctival Sacs,Lacrimal Ducts,Lacrimal Punctum,Lateral Canthus,Medial Canthus,Apparatus, Lacrimal,Apparatus, Nasolacrimal,Canthus, Lateral,Canthus, Medial,Conjunctival Sac,Duct, Lacrimal,Gland, Lacrimal,Lacrimal Duct,Lacrimal Glands,Lacrimal Punctums,Punctum, Lacrimal,Sac, Conjunctival
D008297	Male		Males
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009185	Myelin Proteins	MYELIN-specific proteins that play a structural or regulatory role in the genesis and maintenance of the lamellar MYELIN SHEATH structure.	Myelin Protein,Protein, Myelin,Proteins, Myelin
D011510	Proteolipids	Protein-lipid combinations abundant in brain tissue, but also present in a wide variety of animal and plant tissues. In contrast to lipoproteins, they are insoluble in water, but soluble in a chloroform-methanol mixture. The protein moiety has a high content of hydrophobic amino acids. The associated lipids consist of a mixture of GLYCEROPHOSPHATES; CEREBROSIDES; and SULFOGLYCOSPHINGOLIPIDS; while lipoproteins contain PHOSPHOLIPIDS; CHOLESTEROL; and TRIGLYCERIDES.
D011817	Rabbits	A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes.	Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D004587	Electrophoresis, Agar Gel	Electrophoresis in which agar or agarose gel is used as the diffusion medium.	Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D005260	Female		Females
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia