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A genomic clone containing the gene for the alpha 5 subunit of the human alpha 5 beta 1 integrin complex was isolated by screening a human genomic library with the previously described alpha 5 cDNA (Argraves, W. S., Suzuki, S., Arai, H., Thompson, K., Pierschbacher, M. D., and Ruoslahti, E. (1987) J. Cell Biol 105, 1183-1190). The alpha 5 gene 5'-flanking region lacks both TATA and CCAAT boxes, and it is located in a CpG island. This region was an active promoter in transfection assays using the HT-1080 cell line (fibrosarcoma), which expresses alpha 5, but was inactive in the Raji cell line (B cell), which does not express alpha 5. These results indicate that the alpha 5 gene 5'-flanking region acts as a promoter that exhibits the expected cell-type specificity. Deletion of alpha 5 promoter sequences from position -657 to -178 caused transcription stimulation, suggesting that a silencer is located between these sites. Successive 5' deletions from position -178 decreased promoter activity until activity was essentially eliminated on deletion to position -27. Isolation of a functional promoter for the alpha 5 gene is a first step in understanding how expression of this gene is controlled at the molecular level.
T M Birkenmeier, and
J J McQuillan, and
E D Boedeker, and
W S Argraves, and
E Ruoslahti, and
D C Dean
March 1995,
Biochemical and biophysical research communications,
T M Birkenmeier, and
J J McQuillan, and
E D Boedeker, and
W S Argraves, and
E Ruoslahti, and
D C Dean
January 1994,
Invasion & metastasis,
T M Birkenmeier, and
J J McQuillan, and
E D Boedeker, and
W S Argraves, and
E Ruoslahti, and
D C Dean
September 1991,
Development (Cambridge, England),
T M Birkenmeier, and
J J McQuillan, and
E D Boedeker, and
W S Argraves, and
E Ruoslahti, and
D C Dean
November 1994,
The Journal of cell biology,
T M Birkenmeier, and
J J McQuillan, and
E D Boedeker, and
W S Argraves, and
E Ruoslahti, and
D C Dean
March 1994,
Histopathology,
T M Birkenmeier, and
J J McQuillan, and
E D Boedeker, and
W S Argraves, and
E Ruoslahti, and
D C Dean
November 1994,
Microbiology (Reading, England),
T M Birkenmeier, and
J J McQuillan, and
E D Boedeker, and
W S Argraves, and
E Ruoslahti, and
D C Dean
May 1992,
Biochemical and biophysical research communications,
T M Birkenmeier, and
J J McQuillan, and
E D Boedeker, and
W S Argraves, and
E Ruoslahti, and
D C Dean
September 1996,
Journal of clinical pathology,
T M Birkenmeier, and
J J McQuillan, and
E D Boedeker, and
W S Argraves, and
E Ruoslahti, and
D C Dean
December 2000,
Microbes and infection,
T M Birkenmeier, and
J J McQuillan, and
E D Boedeker, and
W S Argraves, and
E Ruoslahti, and
D C Dean
November 1994,
Research in veterinary science,
