DNA methylation in tumor and matched normal tissues from non-small cell lung cancer patients. 2008

Qinghua Feng, and Stephen E Hawes, and Joshua E Stern, and Linda Wiens, and Hiep Lu, and Zhao Ming Dong, and C Diana Jordan, and Nancy B Kiviat, and Hubert Vesselle
Department of Pathology, School of Medicine, University of Washington, Box 357115, Seattle, WA 98195-7115, USA.

We used MethyLight assays to analyze DNA methylation status of 27 genes on 49 paired cancerous and noncancerous tissue samples from non-small cell lung cancer (NSCLC) patients who underwent surgical resection. Seven genes (RARB, BVES, CDKN2A, KCNH5, RASSF1, CDH13, and RUNX) were found to be methylated significantly more frequently in tumor tissues than in noncancerous tissues. Only methylation of CCND2 and APC was frequently detected in both cancerous and noncancerous tissues, supporting the hypothesis that the methylation of these two genes is a preneoplastic change and may be associated with tobacco smoking exposure. Methylation of any one of eight genes (RASSF1, DAPK1, BVES, CDH13, MGMT, KCNH5, RARB, or CDH1) was present in 80% of NSCLC tissues but only in 14% of noncancerous tissues. Detection of methylation of these genes in blood might have utility in monitoring and detecting tumor recurrence in early-stage NSCLC after curative surgical resection.

UI MeSH Term Description Entries
D008175 Lung Neoplasms Tumors or cancer of the LUNG. Cancer of Lung,Lung Cancer,Pulmonary Cancer,Pulmonary Neoplasms,Cancer of the Lung,Neoplasms, Lung,Neoplasms, Pulmonary,Cancer, Lung,Cancer, Pulmonary,Cancers, Lung,Cancers, Pulmonary,Lung Cancers,Lung Neoplasm,Neoplasm, Lung,Neoplasm, Pulmonary,Pulmonary Cancers,Pulmonary Neoplasm
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D011446 Prospective Studies Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group. Prospective Study,Studies, Prospective,Study, Prospective
D002289 Carcinoma, Non-Small-Cell Lung A heterogeneous aggregate of at least three distinct histological types of lung cancer, including SQUAMOUS CELL CARCINOMA; ADENOCARCINOMA; and LARGE CELL CARCINOMA. They are dealt with collectively because of their shared treatment strategy. Carcinoma, Non-Small Cell Lung,Non-Small Cell Lung Cancer,Non-Small Cell Lung Carcinoma,Non-Small-Cell Lung Carcinoma,Nonsmall Cell Lung Cancer,Carcinoma, Non Small Cell Lung,Carcinomas, Non-Small-Cell Lung,Lung Carcinoma, Non-Small-Cell,Lung Carcinomas, Non-Small-Cell,Non Small Cell Lung Carcinoma,Non-Small-Cell Lung Carcinomas
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000368 Aged A person 65 years of age or older. For a person older than 79 years, AGED, 80 AND OVER is available. Elderly
D014055 Tomography, Emission-Computed Tomography using radioactive emissions from injected RADIONUCLIDES and computer ALGORITHMS to reconstruct an image. CAT Scan, Radionuclide,CT Scan, Radionuclide,Computerized Emission Tomography,Radionuclide Tomography, Computed,Scintigraphy, Computed Tomographic,Tomography, Radionuclide-Computed,Computed Tomographic Scintigraphy,Emission-Computed Tomography,Radionuclide Computer-Assisted Tomography,Radionuclide Computerized Tomography,Radionuclide-Computed Tomography,Radionuclide-Emission Computed Tomography,Tomography, Computerized Emission,CAT Scans, Radionuclide,CT Scans, Radionuclide,Computed Radionuclide Tomography,Computed Tomography, Radionuclide-Emission,Computer-Assisted Tomographies, Radionuclide,Computer-Assisted Tomography, Radionuclide,Computerized Tomography, Radionuclide,Emission Computed Tomography,Emission Tomography, Computerized,Radionuclide CAT Scan,Radionuclide CAT Scans,Radionuclide CT Scan,Radionuclide CT Scans,Radionuclide Computed Tomography,Radionuclide Computer Assisted Tomography,Radionuclide Computer-Assisted Tomographies,Radionuclide Emission Computed Tomography,Scan, Radionuclide CAT,Scan, Radionuclide CT,Scans, Radionuclide CAT,Scans, Radionuclide CT,Tomographic Scintigraphy, Computed,Tomographies, Radionuclide Computer-Assisted,Tomography, Computed Radionuclide,Tomography, Emission Computed,Tomography, Radionuclide Computed,Tomography, Radionuclide Computer-Assisted,Tomography, Radionuclide Computerized,Tomography, Radionuclide-Emission Computed
D015254 DNA Modification Methylases Enzymes that are part of the restriction-modification systems. They are responsible for producing a species-characteristic methylation pattern, on either adenine or cytosine residues, in a specific short base sequence in the host cell's own DNA. This methylated sequence will occur many times in the host-cell DNA and remain intact for the lifetime of the cell. Any DNA from another species which gains entry into a living cell and lacks the characteristic methylation pattern will be recognized by the restriction endonucleases of similar specificity and destroyed by cleavage. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. DNA Modification Methyltransferases,Modification Methylases,Methylases, DNA Modification,Methylases, Modification,Methyltransferases, DNA Modification,Modification Methylases, DNA,Modification Methyltransferases, DNA

Related Publications

Qinghua Feng, and Stephen E Hawes, and Joshua E Stern, and Linda Wiens, and Hiep Lu, and Zhao Ming Dong, and C Diana Jordan, and Nancy B Kiviat, and Hubert Vesselle
March 2010, BMC cancer,
Qinghua Feng, and Stephen E Hawes, and Joshua E Stern, and Linda Wiens, and Hiep Lu, and Zhao Ming Dong, and C Diana Jordan, and Nancy B Kiviat, and Hubert Vesselle
August 2010, Zhongguo fei ai za zhi = Chinese journal of lung cancer,
Qinghua Feng, and Stephen E Hawes, and Joshua E Stern, and Linda Wiens, and Hiep Lu, and Zhao Ming Dong, and C Diana Jordan, and Nancy B Kiviat, and Hubert Vesselle
September 2020, Mutagenesis,
Qinghua Feng, and Stephen E Hawes, and Joshua E Stern, and Linda Wiens, and Hiep Lu, and Zhao Ming Dong, and C Diana Jordan, and Nancy B Kiviat, and Hubert Vesselle
December 2018, Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases,
Qinghua Feng, and Stephen E Hawes, and Joshua E Stern, and Linda Wiens, and Hiep Lu, and Zhao Ming Dong, and C Diana Jordan, and Nancy B Kiviat, and Hubert Vesselle
February 2015, Breast cancer research and treatment,
Qinghua Feng, and Stephen E Hawes, and Joshua E Stern, and Linda Wiens, and Hiep Lu, and Zhao Ming Dong, and C Diana Jordan, and Nancy B Kiviat, and Hubert Vesselle
August 2016, Clinical chemistry and laboratory medicine,
Qinghua Feng, and Stephen E Hawes, and Joshua E Stern, and Linda Wiens, and Hiep Lu, and Zhao Ming Dong, and C Diana Jordan, and Nancy B Kiviat, and Hubert Vesselle
July 2009, Molecular carcinogenesis,
Qinghua Feng, and Stephen E Hawes, and Joshua E Stern, and Linda Wiens, and Hiep Lu, and Zhao Ming Dong, and C Diana Jordan, and Nancy B Kiviat, and Hubert Vesselle
August 2018, The Journal of pathology,
Qinghua Feng, and Stephen E Hawes, and Joshua E Stern, and Linda Wiens, and Hiep Lu, and Zhao Ming Dong, and C Diana Jordan, and Nancy B Kiviat, and Hubert Vesselle
December 2012, Epigenetics,
Qinghua Feng, and Stephen E Hawes, and Joshua E Stern, and Linda Wiens, and Hiep Lu, and Zhao Ming Dong, and C Diana Jordan, and Nancy B Kiviat, and Hubert Vesselle
February 2009, The American journal of pathology,
Copied contents to your clipboard!