BIOMAT Database Logo BIOMAT Database Logo

Acute renal allograft rejection: diagnostic significance of focal peritubular capillary C4d. 2008

Liise K Kayler, and Lawrence Kiss, and Vivek Sharma, and Ravi Mohanka, and Adriana Zeevi, and Alin Girnita, and Ron Shapiro, and Parmjeet S Randhawa
Department of Surgery, University of Florida Medical Center, Gainesville, FL 32610-0286, USA. liisekayler@yahoo.com

BACKGROUND Focal PTC C4d staining in acute renal allograft rejection has not been studied extensively. METHODS Renal allograft biopsies performed after October 2003, representing the first episode of acute rejection (AR) in recipients with > or = 12 months follow-up postbiopsy, were assessed for extent of C4d and correlated with morphology, ELISA screen, donor-specific antibodies (DSA), response to treatment, and outcome. RESULTS In 106 biopsies (16 C4d-diffuse; 24 C4d-focal; 66 C4d-negative), there were no differences among the three groups in terms of timing or grade of AR, creatinine level, tacrolimus level, and grade of interstitial fibrosis/tubular atrophy or graft loss. The C4d-diffuse group was significantly associated with less tubulitis (P=0.0021), and more chronic allograft arteriopathy (P=0.0527). Incomplete response to steroid therapy was more frequent in C4d-diffuse/focal compared with negative cases (P=0.0492). DSA frequency within 1 year of AR was highest in the C4d-diffuse (94%), followed by C4d-focal (38%), and C4d-negative (17%) groups (P<0.0001). CONCLUSIONS Focal PTC C4d was associated with circulating antibodies, with a 2-fold greater diagnostic sensitivity than negative C4d staining. The finding of diffuse C4d on follow-up biopsy was significantly associated with graft loss at 1 year, regardless of index biopsy C4d results.

UI MeSH Term Description Entries
D007684 Kidney Tubules Long convoluted tubules in the nephrons. They collect filtrate from blood passing through the KIDNEY GLOMERULUS and process this filtrate into URINE. Each renal tubule consists of a BOWMAN CAPSULE; PROXIMAL KIDNEY TUBULE; LOOP OF HENLE; DISTAL KIDNEY TUBULE; and KIDNEY COLLECTING DUCT leading to the central cavity of the kidney (KIDNEY PELVIS) that connects to the URETER. Kidney Tubule,Tubule, Kidney,Tubules, Kidney
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D010446 Peptide Fragments Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques. Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D002196 Capillaries The minute vessels that connect arterioles and venules. Capillary Beds,Sinusoidal Beds,Sinusoids,Bed, Sinusoidal,Beds, Sinusoidal,Capillary,Capillary Bed,Sinusoid,Sinusoidal Bed
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260 Female Females
D006084 Graft Rejection An immune response with both cellular and humoral components, directed against an allogeneic transplant, whose tissue antigens are not compatible with those of the recipient. Transplant Rejection,Rejection, Transplant,Transplantation Rejection,Graft Rejections,Rejection, Graft,Rejection, Transplantation,Rejections, Graft,Rejections, Transplant,Rejections, Transplantation,Transplant Rejections,Transplantation Rejections
D006085 Graft Survival The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host. Graft Survivals,Survival, Graft,Survivals, Graft
D006650 Histocompatibility Testing Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. Donor and recipient pairs should be of identical ABO blood group, and in addition should be matched as closely as possible for HISTOCOMPATIBILITY ANTIGENS in order to minimize the likelihood of allograft rejection. (King, Dictionary of Genetics, 4th ed) Crossmatching, Tissue,HLA Typing,Tissue Typing,Crossmatchings, Tissue,HLA Typings,Histocompatibility Testings,Testing, Histocompatibility,Testings, Histocompatibility,Tissue Crossmatching,Tissue Crossmatchings,Tissue Typings,Typing, HLA,Typing, Tissue,Typings, HLA,Typings, Tissue

Related Publications

Liise K Kayler, and Lawrence Kiss, and Vivek Sharma, and Ravi Mohanka, and Adriana Zeevi, and Alin Girnita, and Ron Shapiro, and Parmjeet S Randhawa
Focal peritubular capillary C4d deposition in acute rejection.
May 2006, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,
Liise K Kayler, and Lawrence Kiss, and Vivek Sharma, and Ravi Mohanka, and Adriana Zeevi, and Alin Girnita, and Ron Shapiro, and Parmjeet S Randhawa
Monocytes and peritubular capillary C4d deposition in acute renal allograft rejection.
May 2003, Kidney international,
Liise K Kayler, and Lawrence Kiss, and Vivek Sharma, and Ravi Mohanka, and Adriana Zeevi, and Alin Girnita, and Ron Shapiro, and Parmjeet S Randhawa
Peritubular capillary C4d staining in late acute renal allograft rejection--is it relevant?
January 2008, Clinical transplantation,
Liise K Kayler, and Lawrence Kiss, and Vivek Sharma, and Ravi Mohanka, and Adriana Zeevi, and Alin Girnita, and Ron Shapiro, and Parmjeet S Randhawa
Complement activation in acute humoral renal allograft rejection: diagnostic significance of C4d deposits in peritubular capillaries.
October 1999, Journal of the American Society of Nephrology : JASN,
Liise K Kayler, and Lawrence Kiss, and Vivek Sharma, and Ravi Mohanka, and Adriana Zeevi, and Alin Girnita, and Ron Shapiro, and Parmjeet S Randhawa
Thrombotic microangiopathy and peritubular capillary C4d expression in renal allograft biopsies.
February 2011, Clinical journal of the American Society of Nephrology : CJASN,
Liise K Kayler, and Lawrence Kiss, and Vivek Sharma, and Ravi Mohanka, and Adriana Zeevi, and Alin Girnita, and Ron Shapiro, and Parmjeet S Randhawa
Implications of immunohistochemical detection of C4d along peritubular capillaries in late acute renal allograft rejection.
January 2005, Transplantation,
Liise K Kayler, and Lawrence Kiss, and Vivek Sharma, and Ravi Mohanka, and Adriana Zeevi, and Alin Girnita, and Ron Shapiro, and Parmjeet S Randhawa
C4d/CD34 double-immunofluorescence staining of renal allograft biopsies for assessing peritubular capillary C4d positivity.
March 2012, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc,
Liise K Kayler, and Lawrence Kiss, and Vivek Sharma, and Ravi Mohanka, and Adriana Zeevi, and Alin Girnita, and Ron Shapiro, and Parmjeet S Randhawa
Peritubular capillary C4d deposition in chronic allograft dysfunction.
October 2004, Yonsei medical journal,
Liise K Kayler, and Lawrence Kiss, and Vivek Sharma, and Ravi Mohanka, and Adriana Zeevi, and Alin Girnita, and Ron Shapiro, and Parmjeet S Randhawa
Peritubular capillary C4d deposition in lupus nephritis different from antibody-mediated renal rejection.
January 2007, Lupus,
Liise K Kayler, and Lawrence Kiss, and Vivek Sharma, and Ravi Mohanka, and Adriana Zeevi, and Alin Girnita, and Ron Shapiro, and Parmjeet S Randhawa
Function of CD40/CD40L in peritubular capillary of renal allograft rejection.
January 2001, Transplantation proceedings,
Copied contents to your clipboard!