Ypt1p is essential for retrograde Golgi-ER transport and for Golgi maintenance in S. cerevisiae. 2008

Faustin Kamena, and Melanie Diefenbacher, and Cornelia Kilchert, and Heinz Schwarz, and Anne Spang
Friedrich Miescher Laboratory of the Max Planck Society, Spemannstrasse 39, 72076 Tübingen, Germany.

The small GTPase Ypt1p of the Rab family is required for docking of ER-derived transport vesicles with the Golgi prior to fusion. However, the identity of the Rab protein that mediates docking of Golgi-derived COPI vesicles with the ER in retrograde transport remains elusive. Here, we show that in yeast Ypt1p is essential for retrograde transport from the Golgi to the ER. Retrieval of gpalphaF-HDEL (glycolylated pro-alpha-factor with an HDEL tag at the C-terminus) was blocked in Deltaypt1/SLY1-20 membranes at the restrictive temperature in vitro. Moreover, Ypt1p and the ER-resident t-SNARE Ufe1p interact genetically and biochemically, indicating a role for Ypt1p in consumption of COPI vesicles at the ER. Ypt1p is also essential for the maintenance of the morphology and the protein composition of the Golgi. Interestingly, the concentrations of the Golgi enzymes Anp1p and Mnn1p, the cargo protein Emp47p and the v-SNARE Sec22p were all substantially reduced in Golgi from a Deltaypt1/SLY1-20 strain as compared with wild-type Golgi, while the concentration of Arf1p and of coatomer were mildly affected. Finally, COPI vesicles generated from Deltaypt1/SLY1-20 Golgi membranes in vitro were depleted of Emp47p and Sec22p. These data demonstrate that Ypt1p plays an essential role in retrograde transport from the Golgi to the ER.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D004721 Endoplasmic Reticulum A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed) Ergastoplasm,Reticulum, Endoplasmic
D006056 Golgi Apparatus A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990) Golgi Complex,Apparatus, Golgi,Complex, Golgi
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D020691 rab GTP-Binding Proteins A large family of MONOMERIC GTP-BINDING PROTEINS that play a key role in cellular secretory and endocytic pathways. Rab GTPase,rab G-Proteins,rab GTP-Binding Protein,rab GTPases,G-Proteins, rab,GTP-Binding Protein, rab,GTP-Binding Proteins, rab,GTPase, Rab,GTPases, rab,Protein, rab GTP-Binding,rab G Proteins,rab GTP Binding Protein,rab GTP Binding Proteins
D021381 Protein Transport The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport. Cellular Protein Targeting,Gated Protein Transport,Protein Targeting, Cellular,Protein Translocation,Transmembrane Protein Transport,Vesicular Protein Transport,Protein Localization Processes, Cellular,Protein Sorting,Protein Trafficking,Protein Transport, Gated,Protein Transport, Transmembrane,Protein Transport, Vesicular,Traffickings, Protein
D029701 Saccharomyces cerevisiae Proteins Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes. Baker's Yeast Proteins,S cerevisiae Proteins

Related Publications

Faustin Kamena, and Melanie Diefenbacher, and Cornelia Kilchert, and Heinz Schwarz, and Anne Spang
July 2019, Cells,
Faustin Kamena, and Melanie Diefenbacher, and Cornelia Kilchert, and Heinz Schwarz, and Anne Spang
January 1998, The Journal of cell biology,
Faustin Kamena, and Melanie Diefenbacher, and Cornelia Kilchert, and Heinz Schwarz, and Anne Spang
December 1999, The Journal of cell biology,
Faustin Kamena, and Melanie Diefenbacher, and Cornelia Kilchert, and Heinz Schwarz, and Anne Spang
November 1998, The Journal of cell biology,
Faustin Kamena, and Melanie Diefenbacher, and Cornelia Kilchert, and Heinz Schwarz, and Anne Spang
June 2013, Physiologia plantarum,
Faustin Kamena, and Melanie Diefenbacher, and Cornelia Kilchert, and Heinz Schwarz, and Anne Spang
February 2016, Scientific reports,
Faustin Kamena, and Melanie Diefenbacher, and Cornelia Kilchert, and Heinz Schwarz, and Anne Spang
August 2000, Current opinion in cell biology,
Faustin Kamena, and Melanie Diefenbacher, and Cornelia Kilchert, and Heinz Schwarz, and Anne Spang
August 1998, The Journal of cell biology,
Faustin Kamena, and Melanie Diefenbacher, and Cornelia Kilchert, and Heinz Schwarz, and Anne Spang
November 1999, The Journal of cell biology,
Faustin Kamena, and Melanie Diefenbacher, and Cornelia Kilchert, and Heinz Schwarz, and Anne Spang
October 2014, Biochemical Society transactions,
Copied contents to your clipboard!