-transparent.png){kind=logo}
We investigated whether in vivo excitotoxicity was mediated by a mechanism of programmed cell death called apoptosis. Neurotoxic doses of kainic acid (1.2 nmol) and quinolinic acid (120 nmol) were unilaterally injected in the dorsal hippocampus of anesthetized rats. Eight or 16 h later the animals were killed and DNA was extracted from the injected hippocampi. DNA from mouse thymocytes exposed to methylprednisolone (10(-5) M for 6 h at 37 degrees C) was used as a positive control of apoptotic cells. No typical 'ladder' of DNA fragments (multimers of approximately 200 Kb) which characterizes apoptosis was seen in hippocampal cells after toxic doses of kainic or quinolinic acid, as assessed by agarose gel electrophoresis. This suggests that hippocampal nerve cell death induced in vivo by the excitotoxins is not mediated by apoptosis.
E Ignatowicz, and
A M Vezzani, and
M Rizzi, and
M D'Incalci
June 1988,
Brain research,
E Ignatowicz, and
A M Vezzani, and
M Rizzi, and
M D'Incalci
March 2002,
British journal of pharmacology,
E Ignatowicz, and
A M Vezzani, and
M Rizzi, and
M D'Incalci
January 2018,
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology,
E Ignatowicz, and
A M Vezzani, and
M Rizzi, and
M D'Incalci
January 2002,
Journal of cellular and molecular medicine,
E Ignatowicz, and
A M Vezzani, and
M Rizzi, and
M D'Incalci
February 2008,
The journal of physiological sciences : JPS,
E Ignatowicz, and
A M Vezzani, and
M Rizzi, and
M D'Incalci
January 1989,
Neuroscience letters,
E Ignatowicz, and
A M Vezzani, and
M Rizzi, and
M D'Incalci
January 2019,
Animal cells and systems,
E Ignatowicz, and
A M Vezzani, and
M Rizzi, and
M D'Incalci
July 1987,
Brain research,
E Ignatowicz, and
A M Vezzani, and
M Rizzi, and
M D'Incalci
December 1988,
Experimental neurology,
E Ignatowicz, and
A M Vezzani, and
M Rizzi, and
M D'Incalci
December 2006,
Toxicology in vitro : an international journal published in association with BIBRA,
