-transparent.png){kind=logo}
The alkaline comet assay was tested on different microalgae: the dinoflagellates, Karenia mikimotoi and Alexandrium minutum, and the diatom, Chaetoceros gracilis. The microalgae were exposed during their exponential growth to the model direct genotoxicant, hydrogen peroxide (1h, 5 and 100muM H2O2). Following H2O2 exposure, the comet assay was validated only for K. mikimotoi for which genotoxicity was observed from the lowest tested concentration of 5 microM with a concentration-dependent effect. C. gracilis was too small in size (4 microm) to be correctly analysed. For A. minutum, our lysis buffer was not strong enough to digest the cellulosic thecal plates. For K. mikimotoi, the comet assay was thus applied for the study of the genotoxic effects of different pesticides: epoxiconazole (as Opus formulation), chlorpyriphos-ethyl (as Dursban formulation) and endosulfan at 1, 10 and 100 microg of active substance/L for 24h. Exposure to epoxiconazole in formulation resulted in an increase in the extent of DNA strand breaks at the highest tested concentration icro/L. Endosulfan exposure resulted in DNA damage for K. mikimotoi nuclei. Genotoxicity was observed from 1 microg/L of endosulfan and was not concentration dependent.
F Akcha, and
G Arzul, and
S Rousseau, and
M Bardouil
August 2017,
Ecotoxicology and environmental safety,
F Akcha, and
G Arzul, and
S Rousseau, and
M Bardouil
April 2020,
Environmental monitoring and assessment,
F Akcha, and
G Arzul, and
S Rousseau, and
M Bardouil
January 2001,
Vestnik Rossiiskoi akademii meditsinskikh nauk,
F Akcha, and
G Arzul, and
S Rousseau, and
M Bardouil
July 2002,
Toxicology letters,
F Akcha, and
G Arzul, and
S Rousseau, and
M Bardouil
February 2018,
Saudi journal of biological sciences,
F Akcha, and
G Arzul, and
S Rousseau, and
M Bardouil
May 2010,
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
F Akcha, and
G Arzul, and
S Rousseau, and
M Bardouil
December 2013,
Mutation research. Genetic toxicology and environmental mutagenesis,
F Akcha, and
G Arzul, and
S Rousseau, and
M Bardouil
February 2018,
Physiology and molecular biology of plants : an international journal of functional plant biology,
F Akcha, and
G Arzul, and
S Rousseau, and
M Bardouil
March 2014,
Human & experimental toxicology,
F Akcha, and
G Arzul, and
S Rousseau, and
M Bardouil
September 2013,
Chemosphere,
