Experimental inoculation of house sparrows (Passer domesticus) with buggy creek virus. 2008

Kathryn P Huyvaert, and Amy T Moore, and Nicholas A Panella, and Eric A Edwards, and Mary Bomberger Brown, and Nicholas Komar, and Charles R Brown
Department of Biological Science, University of Tulsa, Tulsa, Oklahoma 74104, USA. Kate.Huyvaert@ColoState.edu

We performed experimental inoculations of house sparrows (Passer domesticus) with Buggy Creek virus (BCRV), a poorly known alphavirus (Togaviridae) vectored primarily by the swallow bug (Hemiptera: Cimicidae: Oeciacus vicarius) that is an ectoparasite of the cliff swallow (Petrochelidon pyrrhonota) and house sparrow. Viremias were detected by plaque assay in two of six birds on days 1-3 postinoculation; viremia was highest on day 2. Viral RNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in blood of six of 12 birds ranging from day 1 to day 15 postinoculation. Infectious BCRV was detected in nasopharyngeal swab samples from two birds by plaque assay. Three control birds that were housed with viremic individuals showed evidence of BCRV RNA in blood (by RT-PCR), suggesting possible bird-to-bird transmission of this virus. Viral RNA also was detected by RT-PCR in brain and skin tissue of six birds on necropsy at the end of the 16-day experiment. Introduced house sparrows are apparently a competent amplifying host for BCRV, and their presence year-round at cliff swallow colonies may facilitate persistence of the virus locally, especially when cliff swallows abandon a site temporarily. The findings that BCRV can be shed orally, that it persists in bird skin, and that control birds could apparently be infected by conspecifics suggest that this virus may be transmitted from bird to bird in the crowded conditions of many cliff swallow colonies.

UI MeSH Term Description Entries
D007303 Insect Vectors Insects that transmit infective organisms from one host to another or from an inanimate reservoir to an animate host. Insect Vector,Vector, Insect,Vectors, Insect
D008297 Male Males
D010948 Viral Plaque Assay Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE. Bacteriophage Plaque Assay,Assay, Bacteriophage Plaque,Assay, Viral Plaque,Assays, Bacteriophage Plaque,Assays, Viral Plaque,Bacteriophage Plaque Assays,Plaque Assay, Bacteriophage,Plaque Assay, Viral,Plaque Assays, Bacteriophage,Plaque Assays, Viral,Viral Plaque Assays
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D005260 Female Females
D006430 Hemiptera A large order of insects characterized by having the mouth parts adapted to piercing or sucking. It is comprised of four suborders: HETEROPTERA, Auchenorrhyncha, Sternorrhyncha, and Coleorrhyncha. Aleurodoidea,Cicadas,Cicadelloidea,Cicadoidea,Coccoidea,Fulgoroidea,Leafhoppers,Psyllids,Psylloidea,Scale Insects,Treehoppers,Whiteflies,Homoptera,Aleurodoideas,Cicada,Cicadelloideas,Cicadoideas,Coccoideas,Fulgoroideas,Hemipteras,Homopteras,Insect, Scale,Insects, Scale,Leafhopper,Psyllid,Psylloideas,Scale Insect,Treehopper,Whitefly
D000524 Alphavirus A genus of TOGAVIRIDAE, also known as Group A arboviruses. They are serologically related to each other and are transmitted by mosquitoes. The type species is the SINDBIS VIRUS. Arboviruses, Group A,Barmah forest virus,Getah virus,Sagiyama virus,Alpha Virus,Alphaviruses,Alpha Viruses,Arbovirus, Group A,Group A Arbovirus,Group A Arboviruses
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000835 Animals, Wild Animals considered to be wild or feral or not adapted for domestic use. It does not include wild animals in zoos for which ANIMALS, ZOO is available. Animals, Nondomestic,Animals, Nondomesticated,Animals, Feral,Stray Animals,Animal, Feral,Animal, Nondomestic,Animal, Nondomesticated,Animal, Stray,Animal, Wild,Animals, Stray,Feral Animal,Feral Animals,Nondomestic Animal,Nondomestic Animals,Nondomesticated Animal,Nondomesticated Animals,Stray Animal,Wild Animal,Wild Animals
D001715 Bird Diseases Diseases of birds not considered poultry, therefore usually found in zoos, parks, and the wild. The concept is differentiated from POULTRY DISEASES which is for birds raised as a source of meat or eggs for human consumption, and usually found in barnyards, hatcheries, etc. Avian Diseases,Avian Disease,Bird Disease,Disease, Avian,Disease, Bird,Diseases, Avian,Diseases, Bird

Related Publications

Kathryn P Huyvaert, and Amy T Moore, and Nicholas A Panella, and Eric A Edwards, and Mary Bomberger Brown, and Nicholas Komar, and Charles R Brown
June 2014, Vector borne and zoonotic diseases (Larchmont, N.Y.),
Kathryn P Huyvaert, and Amy T Moore, and Nicholas A Panella, and Eric A Edwards, and Mary Bomberger Brown, and Nicholas Komar, and Charles R Brown
November 1978, The American journal of tropical medicine and hygiene,
Kathryn P Huyvaert, and Amy T Moore, and Nicholas A Panella, and Eric A Edwards, and Mary Bomberger Brown, and Nicholas Komar, and Charles R Brown
August 1999, Avian pathology : journal of the W.V.P.A,
Kathryn P Huyvaert, and Amy T Moore, and Nicholas A Panella, and Eric A Edwards, and Mary Bomberger Brown, and Nicholas Komar, and Charles R Brown
October 1975, Comparative biochemistry and physiology. A, Comparative physiology,
Kathryn P Huyvaert, and Amy T Moore, and Nicholas A Panella, and Eric A Edwards, and Mary Bomberger Brown, and Nicholas Komar, and Charles R Brown
January 2012, PLoS neglected tropical diseases,
Kathryn P Huyvaert, and Amy T Moore, and Nicholas A Panella, and Eric A Edwards, and Mary Bomberger Brown, and Nicholas Komar, and Charles R Brown
January 2010, Journal of wildlife diseases,
Kathryn P Huyvaert, and Amy T Moore, and Nicholas A Panella, and Eric A Edwards, and Mary Bomberger Brown, and Nicholas Komar, and Charles R Brown
August 2014, Veterinary microbiology,
Kathryn P Huyvaert, and Amy T Moore, and Nicholas A Panella, and Eric A Edwards, and Mary Bomberger Brown, and Nicholas Komar, and Charles R Brown
April 2005, Journal of wildlife diseases,
Kathryn P Huyvaert, and Amy T Moore, and Nicholas A Panella, and Eric A Edwards, and Mary Bomberger Brown, and Nicholas Komar, and Charles R Brown
July 2007, Proceedings. Biological sciences,
Kathryn P Huyvaert, and Amy T Moore, and Nicholas A Panella, and Eric A Edwards, and Mary Bomberger Brown, and Nicholas Komar, and Charles R Brown
August 1966, Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B,
Copied contents to your clipboard!