[Preparation and characterization of monoclonal anti-anti-idiotypic antibody (3D12) of epithelial ovarian carcinoma]. 2008

Chao Zhang, and Heng Cui, and Xiao ping Li, and Xue Ye, and Tian yun Fu, and Jie Feng, and Xiao hong Chang, and Li Hui Wei
Gynecological Oncology Centre, Peking University People's Hospital, Beijing 100044, China.

OBJECTIVE To generate and characterize the anti-anti-idiotypic monoclonal antibody (MAb) of epithelial ovarian carcinoma (EOC)ìwith the aim of further investigating the mechanism of active immunotherapy of tumor vaccine. METHODS Using anti-idiotypic MAb (6B11)of EOC as immunogen, the anti-anti-idiotypic MAb 3D12 was generated by hybridoma technology. To characterize the 3D12, ELISA, immunohistochemistry, flow cytometry and western blot were used to define its specific reactivity with both 6B11 and the original EOC antigen OC166-9. RESULTS One hybridoma cell secreting 3D12 was obtained. The 3D12 showed the ability of specific binding to 6B11 and OC166-9, and of inhibiting the binding of COC166-9 (the MAb against OC166-9) to OC166-9. A positive staining of serous papillary EOC and the specific binding of SKOV3 cell line with 3D12 supernatant were also demonstrated. Western blot showed 3D12 and COC166-9 could immunoprecipitate the same protein. The 3D12 was IgM, with the affinity Kaff approximately 8.34x10(7) L/mol. CONCLUSIONS The anti-anti-idiotypic MAb 3D12 is confirmed as Ab1-like Ab3, which indirectly proves that 6B11 bears the internal image of OC166-9.

UI MeSH Term Description Entries
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D010051 Ovarian Neoplasms Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS. Cancer of Ovary,Ovarian Cancer,Cancer of the Ovary,Neoplasms, Ovarian,Ovary Cancer,Ovary Neoplasms,Cancer, Ovarian,Cancer, Ovary,Cancers, Ovarian,Cancers, Ovary,Neoplasm, Ovarian,Neoplasm, Ovary,Neoplasms, Ovary,Ovarian Cancers,Ovarian Neoplasm,Ovary Cancers,Ovary Neoplasm
D002291 Carcinoma, Papillary A malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. (Stedman, 25th ed) Carcinomas, Papillary,Papillary Carcinoma,Papillary Carcinomas
D005260 Female Females
D006825 Hybridomas Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell. Hybridoma
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000888 Antibodies, Anti-Idiotypic Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies. Anti-Antibodies,Anti-Idiotype Antibodies,Antibodies, Internal Image,Antigamma Globulin Antibodies,Antiglobulins,Anti Antibodies,Anti-gamma Globulin Antibodies,Anti Idiotype Antibodies,Anti gamma Globulin Antibodies,Anti-Idiotypic Antibodies,Antibodies, Anti,Antibodies, Anti Idiotypic,Antibodies, Anti-Idiotype,Antibodies, Anti-gamma Globulin,Antibodies, Antigamma Globulin,Globulin Antibodies, Anti-gamma,Globulin Antibodies, Antigamma,Image Antibodies, Internal,Internal Image Antibodies
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000951 Antigens, Neoplasm Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin. Neoplasm Antigens,Tumor Antigen,Tumor Antigens,Antigen, Tumor,Antigens, Tumor
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

Chao Zhang, and Heng Cui, and Xiao ping Li, and Xue Ye, and Tian yun Fu, and Jie Feng, and Xiao hong Chang, and Li Hui Wei
February 1994, Chinese medical journal,
Chao Zhang, and Heng Cui, and Xiao ping Li, and Xue Ye, and Tian yun Fu, and Jie Feng, and Xiao hong Chang, and Li Hui Wei
August 1996, Zhonghua fu chan ke za zhi,
Chao Zhang, and Heng Cui, and Xiao ping Li, and Xue Ye, and Tian yun Fu, and Jie Feng, and Xiao hong Chang, and Li Hui Wei
April 2003, Hybridoma and hybridomics,
Chao Zhang, and Heng Cui, and Xiao ping Li, and Xue Ye, and Tian yun Fu, and Jie Feng, and Xiao hong Chang, and Li Hui Wei
April 1997, Chinese medical journal,
Chao Zhang, and Heng Cui, and Xiao ping Li, and Xue Ye, and Tian yun Fu, and Jie Feng, and Xiao hong Chang, and Li Hui Wei
January 1989, Zhonghua yi xue za zhi,
Chao Zhang, and Heng Cui, and Xiao ping Li, and Xue Ye, and Tian yun Fu, and Jie Feng, and Xiao hong Chang, and Li Hui Wei
June 1984, Clinical and experimental immunology,
Chao Zhang, and Heng Cui, and Xiao ping Li, and Xue Ye, and Tian yun Fu, and Jie Feng, and Xiao hong Chang, and Li Hui Wei
January 2002, Journal of clinical laboratory analysis,
Chao Zhang, and Heng Cui, and Xiao ping Li, and Xue Ye, and Tian yun Fu, and Jie Feng, and Xiao hong Chang, and Li Hui Wei
December 1995, Seminars in cancer biology,
Chao Zhang, and Heng Cui, and Xiao ping Li, and Xue Ye, and Tian yun Fu, and Jie Feng, and Xiao hong Chang, and Li Hui Wei
June 1996, Zhonghua fu chan ke za zhi,
Chao Zhang, and Heng Cui, and Xiao ping Li, and Xue Ye, and Tian yun Fu, and Jie Feng, and Xiao hong Chang, and Li Hui Wei
January 1985, Current topics in microbiology and immunology,
Copied contents to your clipboard!