-transparent.png){kind=logo}
A monoclonal antibody (1A-LDR1) against sulfidopeptide leukotrienes (LT) is described. The mAb shows a nearly identical detection limit of about 0.04 ng for LTC4, LTD4, LTE4 and NacLTE4 in standard fluid phase RIA. Steric modifications, however, diminish the sensitivity, as determined for the examples 5-epi-LTC4, 6-epi-LTC4, 5,6-epi-LTC4 and 11-trans-LTC4. No crossreactivity could be observed for LTB4. Crossreactions with components of the LT peptide chain such as L-cysteine or glutathione, as well as with arachidonic acid, were not detectable. In assessing the accuracy of the LT-RIA, recovery experiments with supernatants of mouse peritoneal macrophages and incubates of gastric mucosa showed a good correlation of r = 0.993 and 0.990, respectively. Results of an inhibition experiment with mouse peritoneal macrophages, incubated with several concentrations of indomethacin and nordihydroguaiaretic acid (NDGA), support the reliability of RIA and ELISA. The new LT-mAB allows an almost complete detection of peptide leukotrienes in one assay.
M Reinke, and
U Hoppe, and
T Röder, and
H J Bestmann, and
J Mollenhauer, and
K Brune
February 1983,
The Journal of investigative dermatology,
M Reinke, and
U Hoppe, and
T Röder, and
H J Bestmann, and
J Mollenhauer, and
K Brune
January 1991,
Peptides,
M Reinke, and
U Hoppe, and
T Röder, and
H J Bestmann, and
J Mollenhauer, and
K Brune
June 1988,
Prostaglandins,
M Reinke, and
U Hoppe, and
T Röder, and
H J Bestmann, and
J Mollenhauer, and
K Brune
June 1989,
Prostaglandins, leukotrienes, and essential fatty acids,
M Reinke, and
U Hoppe, and
T Röder, and
H J Bestmann, and
J Mollenhauer, and
K Brune
August 1993,
The Journal of pharmacology and experimental therapeutics,
M Reinke, and
U Hoppe, and
T Röder, and
H J Bestmann, and
J Mollenhauer, and
K Brune
August 1983,
Analytical biochemistry,
M Reinke, and
U Hoppe, and
T Röder, and
H J Bestmann, and
J Mollenhauer, and
K Brune
October 1988,
Microcirculation, endothelium, and lymphatics,
M Reinke, and
U Hoppe, and
T Röder, and
H J Bestmann, and
J Mollenhauer, and
K Brune
January 1989,
Revue des maladies respiratoires,
M Reinke, and
U Hoppe, and
T Röder, and
H J Bestmann, and
J Mollenhauer, and
K Brune
November 1990,
The American review of respiratory disease,
M Reinke, and
U Hoppe, and
T Röder, and
H J Bestmann, and
J Mollenhauer, and
K Brune
May 1994,
The European respiratory journal,
