Biomaterial Database

[Polymorphism analysis of 11 Y-STR loci in Manchu population and genetic relationships with 16 populations]. 2008

Mei-Sen Shi, and Ru-Feng Bai, and Jin-Sheng Zhang, and Xiao-Jun Yu

Key Laboratory of Evidence Science (China University of Political Science and Law), Ministry of Education, Beijing 100088, China. shimeisen2000@yahoo.com.cn

To investigate the genetic polymorphisms of 11 Y-chromosomal short tandem repeats(STR) loci in Manchu population, and to evaluate their forensic application values and genetic relationships with 16 other populations of China, 11 Y-STR loci in 203 unrelated Manchu individuals in Liaoning were amplified with PowerPlex Y System, and the PCR products were analyzed by 310 Genetic Analyzer. Cluster analysis and phylogenic trees were applied to show the genetic distance among the populations. As a result, a total of 189 haplotypes were identified and the overall haplotypes diversity for the 11 Y-STR loci was 0.9991. The gene diversity values (GD) for each locus ranged from 0.4594 (DYS391) to 0.9258 (DYS385a/b). Comparing with 16 populations, genetic distance between Manchu and Northeast Han population was the smallest(0.0015), while genetic distance between Man and Uygur ethnic the largest(0.1485). The phylogenetic tree was similar to the results of clustering analysis. The genetic distribution of 11 Y-STR loci in Liaoning Manchu population showed favorable polymorphisms, and was thus suitable for forensic identification and paternity testing in local area. The study of genetic diversity among different populations is useful for the research of their origins, migrations and their relationships.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D011110	Polymorphism, Genetic	The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.	Gene Polymorphism,Genetic Polymorphism,Polymorphism (Genetics),Genetic Polymorphisms,Gene Polymorphisms,Polymorphism, Gene,Polymorphisms (Genetics),Polymorphisms, Gene,Polymorphisms, Genetic
D002681	China	A country spanning from central Asia to the Pacific Ocean.	Inner Mongolia,Manchuria,People's Republic of China,Sinkiang,Mainland China
D006239	Haplotypes	The genetic constitution of individuals with respect to one member of a pair of allelic genes, or sets of genes that are closely linked and tend to be inherited together such as those of the MAJOR HISTOCOMPATIBILITY COMPLEX.	Haplotype
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D020080	Tandem Repeat Sequences	Copies of DNA sequences which lie adjacent to each other in the same orientation (direct tandem repeats) or in the opposite direction to each other (INVERTED TANDEM REPEATS).	Direct Tandem Repeats,Tandem Repeat,Tandem Repeats,Direct Tandem Repeat,Repeat Sequence, Tandem,Repeat Sequences, Tandem,Repeat, Direct Tandem,Repeat, Tandem,Repeats, Direct Tandem,Repeats, Tandem,Sequence, Tandem Repeat,Sequences, Tandem Repeat,Tandem Repeat Sequence,Tandem Repeat, Direct,Tandem Repeats, Direct
D041322	Chromosomes, Human, Y	The human male sex chromosome, being the differential sex chromosome carried by half the male gametes and none of the female gametes in humans.	Y Chromosome, Human,Chromosome, Human Y,Chromosomes, Human Y,Human Y Chromosome,Human Y Chromosomes,Y Chromosomes, Human