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When the patch-clamp technique was used, a slowly activating, time-dependent outward current was identified in both cell-attached and excised membrane patches obtained from guinea pig ventricular myocytes. This macroscopic patch current was present in approximately 50% of patches studied and could be observed both in the presence and absence of unitary single channel activity (i.e., ATP-sensitive K+ channels). The time course of activation of the patch current resembled that of the whole cell delayed-rectifier K+ current (IK) recorded under similar ionic conditions, and the patch current and IK were activated over a similar membrane potential range. The time-dependent patch current could be eliminated when the Nernst potential for K+ equaled that of the pulse voltage. The patch current was inhibited by external addition of the tertiary ammonium compound LY 97241 (50 microM) and was augmented after internal application of the catalytic subunit of adenosine 3',5'-cyclic monophosphate-dependent protein kinase (500 nM). Deactivating tail currents with kinetics similar to those of IK could be recorded to cell-attached and excised patches. Unitary single channel events underlying the time-dependent patch current could not be resolved despite various attempts to increase single channel conductance. Thus our results suggest that a major component of delayed rectification in guinea pig ventricular cells is due to the activity of a high-density, extremely low conductance K+ channel.
K B Walsh, and
J P Arena, and
W M Kwok, and
L Freeman, and
R S Kass
July 1997,
Experimental physiology,
K B Walsh, and
J P Arena, and
W M Kwok, and
L Freeman, and
R S Kass
May 1997,
General pharmacology,
K B Walsh, and
J P Arena, and
W M Kwok, and
L Freeman, and
R S Kass
November 1995,
Zhongguo yao li xue bao = Acta pharmacologica Sinica,
K B Walsh, and
J P Arena, and
W M Kwok, and
L Freeman, and
R S Kass
February 1997,
Nihon Ika Daigaku zasshi,
K B Walsh, and
J P Arena, and
W M Kwok, and
L Freeman, and
R S Kass
January 1994,
Circulation research,
K B Walsh, and
J P Arena, and
W M Kwok, and
L Freeman, and
R S Kass
June 1994,
Circulation research,
K B Walsh, and
J P Arena, and
W M Kwok, and
L Freeman, and
R S Kass
July 1994,
Pflugers Archiv : European journal of physiology,
K B Walsh, and
J P Arena, and
W M Kwok, and
L Freeman, and
R S Kass
May 2003,
Anesthesia and analgesia,
K B Walsh, and
J P Arena, and
W M Kwok, and
L Freeman, and
R S Kass
January 2013,
Evidence-based complementary and alternative medicine : eCAM,
K B Walsh, and
J P Arena, and
W M Kwok, and
L Freeman, and
R S Kass
November 2010,
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology,
