-transparent.png){kind=logo}
The rpsL gene of Escherichia coli was inserted into the BamHI site of transposon Tn5. This transposon was called Tn5-rpsL. Tn5-rpsL may be useful in microbiological studies when one wants to cure various bacterial genera of certain plasmid(s). A streptomycin-resistant (SmR) derivative of the host bacterial strain is first isolated. The plasmid(s) later to be cured are then labelled with Tn5-rpsL, which makes the cells Sm-sensitive. These cells can regain their resistance to Sm if they lose the Tn5-rpsL-tagged plasmid. Thus, plasmid-free bacteria are easily selected among SmR survivors. The frequency of occurrence of the plasmid-less variants of plasmid-containing wild-type Salmonella typhimurium measured by this method is given as an example.
I Stojiljković, and
Z Trgovcević, and
E Salaj-Smic
September 1988,
Proceedings of the National Academy of Sciences of the United States of America,
I Stojiljković, and
Z Trgovcević, and
E Salaj-Smic
January 2008,
Annual review of genetics,
I Stojiljković, and
Z Trgovcević, and
E Salaj-Smic
January 1993,
Annual review of microbiology,
I Stojiljković, and
Z Trgovcević, and
E Salaj-Smic
December 1983,
Proceedings of the National Academy of Sciences of the United States of America,
I Stojiljković, and
Z Trgovcević, and
E Salaj-Smic
January 1989,
Doklady Akademii nauk SSSR,
I Stojiljković, and
Z Trgovcević, and
E Salaj-Smic
September 1993,
Plasmid,
I Stojiljković, and
Z Trgovcević, and
E Salaj-Smic
December 1983,
Genetics,
I Stojiljković, and
Z Trgovcević, and
E Salaj-Smic
December 1988,
Canadian journal of microbiology,
I Stojiljković, and
Z Trgovcević, and
E Salaj-Smic
September 1984,
Molecular biology and evolution,
I Stojiljković, and
Z Trgovcević, and
E Salaj-Smic
November 1987,
Infection and immunity,
