PAX3/7 expression coincides with MyoD during chronic skeletal muscle overload. 2008

Jon-Philippe K Hyatt, and Gary E McCall, and Elizabeth M Kander, and Hui Zhong, and Roland R Roy, and Kimberly A Huey
Department of Human Science, School of Nursing and Health Studies, Georgetown University, 3700 Reservoir Rd., Washington, DC 20057, USA. jkh22@georgetown.edu

Paired box (Pax) proteins 3 and 7 are key determinants for embryonic skeletal muscle development by initiating myogenic regulatory factor (MRF) gene expression. We show that Pax3 and 7 participate in adult skeletal muscle plasticity during the initial responses to chronic overload (< or =7 days) and appear to coordinate MyoD expression, a member of the MRF family of genes. Pax3 and 7 mRNA were higher than control within 12 h after initiation of overload, preceded the increase in MyoD mRNA on day 1, and peaked on day 2. On days 3 and 7, Pax7 mRNA remained higher than control, suggesting that satellite cell self-renewal was occurring. Pax3 and 7 and MyoD protein levels were higher than control on days 2 and 3. These data indicate that Pax3 and 7 coordinate the recapitulation of developmental-like regulatory mechanisms in response to growth-inducing stimuli in adult skeletal muscle, presumably through activation of satellite cells.

UI MeSH Term Description Entries
D009124 Muscle Proteins The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN. Muscle Protein,Protein, Muscle,Proteins, Muscle
D009929 Organ Size The measurement of an organ in volume, mass, or heaviness. Organ Volume,Organ Weight,Size, Organ,Weight, Organ
D001835 Body Weight The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms. Body Weights,Weight, Body,Weights, Body
D005260 Female Females
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D000072041 PAX3 Transcription Factor A paired box transcription factor that functions in CELL PROLIFERATION; CELL MIGRATION; and APOPTOSIS. It also is involved in MYOGENESIS and neural development. Mutations in the PAX3 gene are associated with WAARDENBURG SYNDROME TYPE 1. Paired Box 3 Protein,Paired Box Transcription Factor 3,Transcription Factor PAX3,Factor PAX3, Transcription,PAX3, Transcription Factor,Transcription Factor, PAX3
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats

Related Publications

Jon-Philippe K Hyatt, and Gary E McCall, and Elizabeth M Kander, and Hui Zhong, and Roland R Roy, and Kimberly A Huey
January 2011, Molecular biology reports,
Jon-Philippe K Hyatt, and Gary E McCall, and Elizabeth M Kander, and Hui Zhong, and Roland R Roy, and Kimberly A Huey
February 2009, Zhonghua yi xue za zhi,
Jon-Philippe K Hyatt, and Gary E McCall, and Elizabeth M Kander, and Hui Zhong, and Roland R Roy, and Kimberly A Huey
October 2004, Genes & development,
Jon-Philippe K Hyatt, and Gary E McCall, and Elizabeth M Kander, and Hui Zhong, and Roland R Roy, and Kimberly A Huey
December 2007, The Journal of biological chemistry,
Jon-Philippe K Hyatt, and Gary E McCall, and Elizabeth M Kander, and Hui Zhong, and Roland R Roy, and Kimberly A Huey
February 1998, Molecular and cellular biology,
Jon-Philippe K Hyatt, and Gary E McCall, and Elizabeth M Kander, and Hui Zhong, and Roland R Roy, and Kimberly A Huey
May 2003, Molecular biology of the cell,
Jon-Philippe K Hyatt, and Gary E McCall, and Elizabeth M Kander, and Hui Zhong, and Roland R Roy, and Kimberly A Huey
January 2012, Genes & development,
Jon-Philippe K Hyatt, and Gary E McCall, and Elizabeth M Kander, and Hui Zhong, and Roland R Roy, and Kimberly A Huey
March 2015, Genes & development,
Jon-Philippe K Hyatt, and Gary E McCall, and Elizabeth M Kander, and Hui Zhong, and Roland R Roy, and Kimberly A Huey
May 1999, The International journal of developmental biology,
Jon-Philippe K Hyatt, and Gary E McCall, and Elizabeth M Kander, and Hui Zhong, and Roland R Roy, and Kimberly A Huey
February 2013, The Journal of experimental biology,
Copied contents to your clipboard!