Biomaterial Database

Infection of the midgut of Culicoides variipennis (Diptera: Ceratopogonidae) with bluetongue virus. 1991

P J Sieburth, and C E Nunamaker, and J Ellis, and R A Nunamaker

Arthropod-borne Animal Diseases Research Laboratory, USDA-ARS, University Station, Laramie, Wyoming 82071.

When Culicoides variipennis (Coquillett) ingested a bluetongue virus (BTV)-defibrinated sheep blood suspension, BTV adsorbed to sheep red blood cells (RBCs) within 2 h. The virus had entered RBCs by 6 h and was still seen in RBCs 2 d after ingestion of the blood meal, even though the RBCs had been dehydrated. The peritrophic membrane began to form on day 1, and it contained breaks by day 3. The peritrophic membrane did not prevent infection of the midgut epithelium. Viral replication occurred in the midgut cells; virions matured through the basolateral extracellular membrane into the extracellular spaces between the plasma membrane and the basal lamina. The greatest number of virions was seen on day 3. The virus did not accumulate but rather exited the cells when mature. No cytopathology was observed in virus-infected cells, and midgut cells became vacuolated and sloughed off into the midgut lumen by day 3 in both control and virus-infected cells.

UI	MeSH Term	Description	Entries
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D001820	Bluetongue virus	The type species of ORBIVIRUS causing a serious disease in sheep, especially lambs. It may also infect wild ruminants and other domestic animals.	Ovine Catarrhal Fever Virus,Blue Tongue Virus,Blue Tongue Viruses,Bluetongue Viruses
D002519	Ceratopogonidae	A family of biting midges, in the order DIPTERA. It includes the genus Culicoides which transmits filarial parasites pathogenic to man and other primates.	Culicoides,Leptoconops,Midges, Biting,Biting Midge,Biting Midges,Culicoide,Leptoconop,Midge, Biting
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012756	Sheep	Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.	Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D014771	Virion	The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.	Virus Particle,Viral Particle,Viral Particles,Particle, Viral,Particle, Virus,Particles, Viral,Particles, Virus,Virions,Virus Particles