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Recent studies suggest that different subtypes of receptors may mediate the action of various bombesin-related peptides in different tissues. In the present study the ability of bombesin and its structurally related peptides [litorin, gastrin-releasing peptide (GRP), GRP18-27, neuromedin B, [Leu8]litorin, and bombesin nonapeptide BN(6-14)] to interact with smooth muscle cells isolated from guinea pig stomach was investigated. Each peptide induced a specific contractile response with potencies (D50 in pM) of [Leu8]litorin (0.7) greater than bombesin (1.2) greater than litorin (3) greater than neuromedin B (3.5) = GRP (3.8) = GRP18-27 (3.9) greater than BN(6-14) (70.9). The specific bombesin receptor antagonist psi 13,14-bombesin differed in its potency for inhibiting equipotent concentrations of bombesin, GRP, or neuromedin B, was equipotent for bombesin or GRP (IC50 12.7 and 22.1 nM), and was 11 times less potent for neuromedin B (IC50 234.5 nM), suggesting the presence of subtypes of receptors mediating the action of bombesin-related peptides. To further investigate this possibility, a technique of receptor protection that enables selective preservation of one receptor type was used. GRP or bombesin protected completely the response to GRP or bombesin but abolished the subsequent contractile response to neuromedin B. Neuromedin B, instead, protected only the response to neuromedin B. These results demonstrate that gastric smooth muscle cells possess specific receptors that interact with bombesin-related peptides and that two receptor subtypes mediate the contractile response to these peptides: one subtype is selective for bombesin or GRP, the other for neuromedin B.
C Severi, and
R T Jensen, and
V Erspamer, and
L D'Arpino, and
D H Coy, and
A Torsoli, and
G Delle Fave
August 1994,
Regulatory peptides,
C Severi, and
R T Jensen, and
V Erspamer, and
L D'Arpino, and
D H Coy, and
A Torsoli, and
G Delle Fave
November 1997,
Pflugers Archiv : European journal of physiology,
C Severi, and
R T Jensen, and
V Erspamer, and
L D'Arpino, and
D H Coy, and
A Torsoli, and
G Delle Fave
February 1988,
The American journal of physiology,
C Severi, and
R T Jensen, and
V Erspamer, and
L D'Arpino, and
D H Coy, and
A Torsoli, and
G Delle Fave
January 1988,
Annals of the New York Academy of Sciences,
C Severi, and
R T Jensen, and
V Erspamer, and
L D'Arpino, and
D H Coy, and
A Torsoli, and
G Delle Fave
June 1988,
The American journal of physiology,
C Severi, and
R T Jensen, and
V Erspamer, and
L D'Arpino, and
D H Coy, and
A Torsoli, and
G Delle Fave
April 1988,
Nihon Hinyokika Gakkai zasshi. The japanese journal of urology,
C Severi, and
R T Jensen, and
V Erspamer, and
L D'Arpino, and
D H Coy, and
A Torsoli, and
G Delle Fave
September 1982,
Die Pharmazie,
C Severi, and
R T Jensen, and
V Erspamer, and
L D'Arpino, and
D H Coy, and
A Torsoli, and
G Delle Fave
January 2000,
Hypertension (Dallas, Tex. : 1979),
C Severi, and
R T Jensen, and
V Erspamer, and
L D'Arpino, and
D H Coy, and
A Torsoli, and
G Delle Fave
January 1991,
Digestive diseases (Basel, Switzerland),
C Severi, and
R T Jensen, and
V Erspamer, and
L D'Arpino, and
D H Coy, and
A Torsoli, and
G Delle Fave
May 1982,
Nature,
