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Chemical synthesis of a series of modified oligodeoxyribonucleotides containing one or two residues of thymidine glycol (5,6-dihydro-5,6-dihydroxythymidine), the main product of oxidative DNA damage, is described. The thermal stability of DNA duplexes containing thymidine glycol residues was studied using UV spectroscopy. Introduction of even one thymidine glycol residue into the duplex structure was shown to result in its significant destabilization. Data on the interaction of DNA methyltransferases and type II restriction endonucleases with DNA ligands containing oxidized thymine were obtained for the first time. Introduction of a thymidine glycol residue into the central degenerate position of the recognition site of restriction endonuclease SsoII was found to result in an increase in the initial hydrolysis rate of the modified duplex in comparison with that of the unmodified structure. The affinity of C5-cytosine methyltransferase SsoII for the DNA duplex bearing thymidine glycol was found to be twofold higher than for the unmodified substrate. However, such a modification of the DNA ligand prevents its methylation. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2008, vol. 34, no. 2; see also http://www.maik.ru.
E A Fedotova, and
F Ian, and
E A Kubareva, and
E A Romanova, and
A S Protsenko, and
M B Viriasov, and
T Hianik, and
T S Oretskaia
January 2005,
Nucleosides, nucleotides & nucleic acids,
E A Fedotova, and
F Ian, and
E A Kubareva, and
E A Romanova, and
A S Protsenko, and
M B Viriasov, and
T Hianik, and
T S Oretskaia
December 1984,
Nucleic acids research,
E A Fedotova, and
F Ian, and
E A Kubareva, and
E A Romanova, and
A S Protsenko, and
M B Viriasov, and
T Hianik, and
T S Oretskaia
April 2007,
Biochemistry,
E A Fedotova, and
F Ian, and
E A Kubareva, and
E A Romanova, and
A S Protsenko, and
M B Viriasov, and
T Hianik, and
T S Oretskaia
January 2014,
PloS one,
E A Fedotova, and
F Ian, and
E A Kubareva, and
E A Romanova, and
A S Protsenko, and
M B Viriasov, and
T Hianik, and
T S Oretskaia
January 2015,
PloS one,
E A Fedotova, and
F Ian, and
E A Kubareva, and
E A Romanova, and
A S Protsenko, and
M B Viriasov, and
T Hianik, and
T S Oretskaia
October 2011,
Bioorganic & medicinal chemistry,
E A Fedotova, and
F Ian, and
E A Kubareva, and
E A Romanova, and
A S Protsenko, and
M B Viriasov, and
T Hianik, and
T S Oretskaia
August 1991,
Nucleic acids research,
E A Fedotova, and
F Ian, and
E A Kubareva, and
E A Romanova, and
A S Protsenko, and
M B Viriasov, and
T Hianik, and
T S Oretskaia
February 1997,
Biochemistry,
E A Fedotova, and
F Ian, and
E A Kubareva, and
E A Romanova, and
A S Protsenko, and
M B Viriasov, and
T Hianik, and
T S Oretskaia
January 1985,
Progress in clinical and biological research,
E A Fedotova, and
F Ian, and
E A Kubareva, and
E A Romanova, and
A S Protsenko, and
M B Viriasov, and
T Hianik, and
T S Oretskaia
January 2005,
Nucleic acids symposium series (2004),
