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A method is described for the radioimmunoassay of 18-OH-DOC using antibodies generated in rabbits against the carboxymethoxime derivative coupled to bovine serum albumin. The procedure uses 4 ml of plasma with intra and interassay variations of 8 and 9% respectively. Standard 18-OH-DOC added to plasma from an adrenalectomized patient gave a regression equation, Y=0.974X+/-2.210 and a correlation coefficient of 0.999. The only cross reacting steroid, 18-OH-B which may lead to falsely high levels is removed by a single thin layer chromatographic step. Blood levels in normal subjects agree closely with those calculated indirectly by metabolic clearance and secretion rate measurements. ACTH stimulation produced an 18-fold increase in plasma concentration while dexamethasone suppression decreased levels 3-fold. Four hours in the upright position resulted in a decreased plasma concentration while aldosterone increased. No significant response to dietary sodium restriction could be demonstrated.
S L Dale, and
P Komanicky, and
J H Pratt, and
J C Melby
April 1978,
Endocrinologia japonica,
S L Dale, and
P Komanicky, and
J H Pratt, and
J C Melby
March 1978,
Journal of steroid biochemistry,
S L Dale, and
P Komanicky, and
J H Pratt, and
J C Melby
October 1979,
Clinical endocrinology,
S L Dale, and
P Komanicky, and
J H Pratt, and
J C Melby
August 1977,
Nihon Naibunpi Gakkai zasshi,
S L Dale, and
P Komanicky, and
J H Pratt, and
J C Melby
April 1978,
The Tohoku journal of experimental medicine,
S L Dale, and
P Komanicky, and
J H Pratt, and
J C Melby
July 1976,
The Journal of clinical investigation,
S L Dale, and
P Komanicky, and
J H Pratt, and
J C Melby
June 1978,
Endocrinologia japonica,
S L Dale, and
P Komanicky, and
J H Pratt, and
J C Melby
February 1996,
Internal medicine (Tokyo, Japan),
S L Dale, and
P Komanicky, and
J H Pratt, and
J C Melby
May 1977,
Mayo Clinic proceedings,
S L Dale, and
P Komanicky, and
J H Pratt, and
J C Melby
February 1976,
Steroids,
