[Effect of Tityus discrepans scorpion venom on global coagulation test. Preliminary studies]. 2008

Josmary Brazón, and Belsy Guerrero, and Carmen Luisa Arocha-Piñango, and Carlos Sevcik, and Gina D'Suze
Laboratorio de Neurofarmacología Celular, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Apartado 21827, Caracas 1020-A, Venezuela.

Tityus discrepans (Td) scorpion venom modifies clotting times in humans. We studied the in vitro venom effect on partial thromboplastin time (PTT), prothrombin time (PT) and its direct clotting activity, using fresh human plasma and purified fibrinogen as substrates. Whole venom (WV) was fractioned with a Protein Pak 125 molecular exclusion column (0.5 mL/min, CH3COONH4 20 mM, pH 4.7). Six fractions (F1 through F6) with retention times ranging from 12.8 to 31 min were obtained. WV (78 to 625 microg/mL) and fraction F1 (10 to 42.5 microg/mL), shortened PTT; WV (700 to 1000 microg/mL) and fraction F6 (16.5 to 700 microg/mL), prolonged PTT. WV (40 to 240 microg/mL) and fraction F2 (5 to 40 microg/mL), prolonged PT. No thrombin-like activity was found with this venom on human plasma or purified fibrinogen. Td venom contains procoagulant components, able to shorten PTT. In addition, the venom contains anticoagulant components which prolong PT and PTT.

UI MeSH Term Description Entries
D010314 Partial Thromboplastin Time The time required for the appearance of FIBRIN strands following the mixing of PLASMA with phospholipid platelet substitute (e.g., crude cephalins, soybean phosphatides). It is a test of the intrinsic pathway (factors VIII, IX, XI, and XII) and the common pathway (fibrinogen, prothrombin, factors V and X) of BLOOD COAGULATION. It is used as a screening test and to monitor HEPARIN therapy. Activated Partial Thromboplastin Time,Cephalin-Kaolin Coagulation Time,Kaolin-Cephalin Coagulation Time,Thromboplastin Time, Partial,Coagulation Time, Cephalin-Kaolin,Cephalin Kaolin Coagulation Time,Coagulation Time, Cephalin Kaolin,Coagulation Time, Kaolin-Cephalin,Kaolin Cephalin Coagulation Time
D011517 Prothrombin Time Clotting time of PLASMA recalcified in the presence of excess TISSUE THROMBOPLASTIN. Factors measured are FIBRINOGEN; PROTHROMBIN; FACTOR V; FACTOR VII; and FACTOR X. It is used for monitoring anticoagulant therapy with COUMARINS. Quick Test,Russell's Viper Venom Time,Thrombotest,Russell Viper Venom Time,Russells Viper Venom Time,Prothrombin Times,Test, Quick,Time, Prothrombin,Times, Prothrombin
D001777 Blood Coagulation The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot. Blood Clotting,Coagulation, Blood,Blood Clottings,Clotting, Blood
D001780 Blood Coagulation Tests Laboratory tests for evaluating the individual's clotting mechanism. Coagulation Tests, Blood,Tests, Blood Coagulation,Blood Coagulation Test,Coagulation Test, Blood,Test, Blood Coagulation
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012604 Scorpion Venoms Venoms from animals of the order Scorpionida of the class Arachnida. They contain neuro- and hemotoxins, enzymes, and various other factors that may release acetylcholine and catecholamines from nerve endings. Of the several protein toxins that have been characterized, most are immunogenic. Scorpion Toxin,Scorpion Toxins,Scorpion Venom Peptide,Tityus serrulatus Venom,Scorpion Venom,alpha-Scorpion Toxin,beta-Scorpion Toxin,gamma-Scorpion Toxin,Peptide, Scorpion Venom,Toxin, Scorpion,Toxin, alpha-Scorpion,Toxin, beta-Scorpion,Venom Peptide, Scorpion,Venom, Scorpion,Venom, Tityus serrulatus,alpha Scorpion Toxin,beta Scorpion Toxin,gamma Scorpion Toxin

Related Publications

Josmary Brazón, and Belsy Guerrero, and Carmen Luisa Arocha-Piñango, and Carlos Sevcik, and Gina D'Suze
March 2013, Toxicon : official journal of the International Society on Toxinology,
Josmary Brazón, and Belsy Guerrero, and Carmen Luisa Arocha-Piñango, and Carlos Sevcik, and Gina D'Suze
July 2006, Parasitology research,
Josmary Brazón, and Belsy Guerrero, and Carmen Luisa Arocha-Piñango, and Carlos Sevcik, and Gina D'Suze
July 1999, FEBS letters,
Josmary Brazón, and Belsy Guerrero, and Carmen Luisa Arocha-Piñango, and Carlos Sevcik, and Gina D'Suze
March 2004, Toxicon : official journal of the International Society on Toxinology,
Josmary Brazón, and Belsy Guerrero, and Carmen Luisa Arocha-Piñango, and Carlos Sevcik, and Gina D'Suze
December 2007, Toxicon : official journal of the International Society on Toxinology,
Josmary Brazón, and Belsy Guerrero, and Carmen Luisa Arocha-Piñango, and Carlos Sevcik, and Gina D'Suze
December 1997, Toxicon : official journal of the International Society on Toxinology,
Josmary Brazón, and Belsy Guerrero, and Carmen Luisa Arocha-Piñango, and Carlos Sevcik, and Gina D'Suze
December 2004, Toxicon : official journal of the International Society on Toxinology,
Josmary Brazón, and Belsy Guerrero, and Carmen Luisa Arocha-Piñango, and Carlos Sevcik, and Gina D'Suze
June 2006, Proteomics,
Josmary Brazón, and Belsy Guerrero, and Carmen Luisa Arocha-Piñango, and Carlos Sevcik, and Gina D'Suze
December 2010, Toxicon : official journal of the International Society on Toxinology,
Josmary Brazón, and Belsy Guerrero, and Carmen Luisa Arocha-Piñango, and Carlos Sevcik, and Gina D'Suze
January 2011, Platelets,
Copied contents to your clipboard!