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To evaluate the effects of P. amarus on hepatitis B virus (HBV) antigens and HBV-DNA, initial ethanolic extract and subsequent fractions of the plants were prepared. The whole plant material was dried, powdered and extracted with alcohol and subsequently fractionated in hexane, chloroform, butanol and finally in water. All the material were tested for in vitro effects on HBsAg, HBeAg and HBV-DNA in serum samples positive for HBV antigens followed by the screening of respective antigens by Elisa. HBV-DNA was determined by molecular hybridization. The extracts were effective against HBV antigens, the butanol extract being the most potent. Further chromatographic fractions showed an enhanced activity. The active fractions inhibited the interaction between HBsAg/HBeAg and their corresponding antibodies suggesting anti-HBs, anti-HBe-like activity and also an effect on HBV-DNA.
R Mehrotra, and
S Rawat, and
D K Kulshreshtha, and
P Goyal, and
G K Patnaik, and
B N Dhawan
October 1988,
Lancet (London, England),
R Mehrotra, and
S Rawat, and
D K Kulshreshtha, and
P Goyal, and
G K Patnaik, and
B N Dhawan
October 1988,
Lancet (London, England),
R Mehrotra, and
S Rawat, and
D K Kulshreshtha, and
P Goyal, and
G K Patnaik, and
B N Dhawan
December 1990,
Journal of medical virology,
R Mehrotra, and
S Rawat, and
D K Kulshreshtha, and
P Goyal, and
G K Patnaik, and
B N Dhawan
October 1990,
Lancet (London, England),
R Mehrotra, and
S Rawat, and
D K Kulshreshtha, and
P Goyal, and
G K Patnaik, and
B N Dhawan
December 1996,
European journal of clinical investigation,
R Mehrotra, and
S Rawat, and
D K Kulshreshtha, and
P Goyal, and
G K Patnaik, and
B N Dhawan
September 1991,
Journal of the Medical Association of Thailand = Chotmaihet thangphaet,
R Mehrotra, and
S Rawat, and
D K Kulshreshtha, and
P Goyal, and
G K Patnaik, and
B N Dhawan
January 1989,
Cancer detection and prevention,
R Mehrotra, and
S Rawat, and
D K Kulshreshtha, and
P Goyal, and
G K Patnaik, and
B N Dhawan
September 1997,
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology,
R Mehrotra, and
S Rawat, and
D K Kulshreshtha, and
P Goyal, and
G K Patnaik, and
B N Dhawan
March 1990,
Vaccine,
R Mehrotra, and
S Rawat, and
D K Kulshreshtha, and
P Goyal, and
G K Patnaik, and
B N Dhawan
December 1993,
Journal of medical virology,
