BIOMAT Database Logo BIOMAT Database Logo

Comparison of a slide agglutination test, LeptoTek Dri-Dot, and IgM-ELISA with microscopic agglutination test for Leptospira antibody detection. 2007

Chintana Chirathaworn, and Yada Kaewopas, and Yong Poovorawan, and Duangjai Suwancharoen
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. chintana_chula@yahoo.co.th

A slide agglutination test (SAT), LeptoTek Dri-Dot and IgM-ELISA were compared with a microscopic agglutination test (MAT) for the detection of Leptospira antibodies. Paired sera from 10 patients whose leptospirosis was clinically suspected and diagnosed by MAT, were evaluated in this study. Our data, especially from acute samples, demonstrate the SAT and Dri-Dot were more sensitive as initial screening tests than MAT. IgM-ELISA has an advantage over MAT, SAT, and Dri-Dot since the results can be interpreted from a single serum testing if the results of the test are positive. Eight of the ten cases could be diagnosed by IgM-ELISA. Our data suggest that IgM-ELISA may be used for the diagnosis of leptospirosis. However, the agglutination test is useful for screening and for secondary infection cases for which IgM antibodies may be undetectable. MAT can be performed as a reference test and when information regarding the causative serovar is required.

UI MeSH Term Description Entries
D007075 Immunoglobulin M A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally was called a macroglobulin. Gamma Globulin, 19S,IgM,IgM Antibody,IgM1,IgM2,19S Gamma Globulin,Antibody, IgM
D007919 Leptospira A genus of aerobic, helical spirochetes, some species of which are pathogenic, others free-living or saprophytic.
D007922 Leptospirosis Infections with bacteria of the genus LEPTOSPIRA. Cane-Cutter Fever,Canicola Fever,Leptospira Canicola Infection,Leptospira Infection,Leptospirosis Canicola,Mud Fever,Rice-Field Fever,Stuttgart Disease,Swineherd's Disease,Cane Cutter Fever,Fever, Canicola,Fever, Mud,Fever, Rice-Field,Fevers, Cane-Cutter,Infection, Leptospira,Infection, Leptospira Canicola,Infections, Leptospira,Infections, Leptospira Canicola,Leptospira Canicola Infections,Leptospira Infections,Leptospiroses,Leptospirosis Canicolas,Rice Field Fever,Swineherd's Diseases
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000372 Agglutination Tests Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed) Agglutination Test,Test, Agglutination,Tests, Agglutination
D000907 Antibodies, Bacterial Immunoglobulins produced in a response to BACTERIAL ANTIGENS. Bacterial Antibodies
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity

Related Publications

Chintana Chirathaworn, and Yada Kaewopas, and Yong Poovorawan, and Duangjai Suwancharoen
Rapid serodiagnosis of leptospirosis using the IgM-specific Dot-ELISA: comparison with the microscopic agglutination test.
March 1985, The American journal of tropical medicine and hygiene,
Chintana Chirathaworn, and Yada Kaewopas, and Yong Poovorawan, and Duangjai Suwancharoen
Diagnosis of Leptospirosis: Comparison between Microscopic Agglutination Test, IgM-ELISA and IgM Rapid Immunochromatography Test.
January 2015, PloS one,
Chintana Chirathaworn, and Yada Kaewopas, and Yong Poovorawan, and Duangjai Suwancharoen
A comparison of the rapid macroscopic slide agglutination test with the microscopic slide agglutination test for leptospirosis.
January 1964, Proceedings, annual meeting of the United States Animal Health Association,
Chintana Chirathaworn, and Yada Kaewopas, and Yong Poovorawan, and Duangjai Suwancharoen
Evaluation of IgM LAT and IgM ELISA as compared to microscopic agglutination test (MAT) for early diagnosis of Leptospira sp.
December 2019, Tropical biomedicine,
Chintana Chirathaworn, and Yada Kaewopas, and Yong Poovorawan, and Duangjai Suwancharoen
A comparison of the rapid macroscopic slide agglutination test with the microscopic agglutination test for leptospirosis.
April 1967, The Cornell veterinarian,
Chintana Chirathaworn, and Yada Kaewopas, and Yong Poovorawan, and Duangjai Suwancharoen
A latex slide agglutination test for rapid detection of antimyeloperoxidase antibody.
October 1999, Journal of clinical pathology,
Chintana Chirathaworn, and Yada Kaewopas, and Yong Poovorawan, and Duangjai Suwancharoen
Diagnosis of Human Leptospirosis: Comparison of Microscopic Agglutination Test with Recombinant LigA/B Antigen-Based In-House IgM Dot ELISA Dipstick Test and Latex Agglutination Test Using Bayesian Latent Class Model and MAT as Gold Standard.
June 2022, Diagnostics (Basel, Switzerland),
Chintana Chirathaworn, and Yada Kaewopas, and Yong Poovorawan, and Duangjai Suwancharoen
Comparison of fluorescent antibody and microscopic agglutination testing for Leptospira in pregnant and nonpregnant cows.
January 2010, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
Chintana Chirathaworn, and Yada Kaewopas, and Yong Poovorawan, and Duangjai Suwancharoen
Comparison of the Serion IgM ELISA and Microscopic Agglutination Test for diagnosis of Leptospira spp. infections in sera from different geographical origins and estimation of Leptospira seroprevalence in the Wiwa indigenous population from Colombia.
June 2022, PLoS neglected tropical diseases,
Chintana Chirathaworn, and Yada Kaewopas, and Yong Poovorawan, and Duangjai Suwancharoen
Development and Standardization of Dot - ELISA for Detection of Neospora caninum Antibodies in Cattle and Comparison with Standard Indirect ELISA and Direct Agglutination Test (DAT).
October 2013, Iranian journal of parasitology,
Copied contents to your clipboard!