Biomaterial Database

Molecular epidemiology of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from global clinical trials. 2008

Richard V Goering, and Ribhi M Shawar, and Nicole E Scangarella, and F Patrick O'Hara, and Heather Amrine-Madsen, and Joshua M West, and Marybeth Dalessandro, and Julie A Becker, and Stacey L Walsh, and Linda A Miller, and Stephanie F van Horn, and Elizabeth S Thomas, and Monique E Twynholm

Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, NE 68178, USA. rgoering@creighton.edu

Determining the genetic characteristics of Staphylococcus aureus is important for better understanding of the global and dynamic epidemiology of this organism as we witness the emergence and spread of virulent and antibiotic-resistant clones. We genotyped 292 S. aureus isolates (105 methicillin resistant and 187 methicillin susceptible) using a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and SCCmec typing. In addition, S. aureus isolates were tested for the presence of the Panton-Valentine leukocidin (PVL) genes. Isolates were recovered from patients with uncomplicated skin infections in 10 different countries during five phase III global clinical trials of retapamulin, a new topical antibiotic agent. The most common methicillin-resistant clone had multilocus sequence type 8, pulsed-field type USA300, and SCCmec type IV and possessed the PVL genes. This clone was isolated exclusively in the United States. The most common PVL-positive, methicillin-susceptible clone had multilocus sequence type 121 and pulsed-field type USA1200. This clone was found primarily in South Africa and the Russian Federation. Other clones were found at lower frequencies and were limited in their geographic distribution. Overall, considerable genetic diversity was observed within multilocus sequence type clonal complexes and pulsed-field types.

UI	MeSH Term	Description	Entries
D007194	India	A country in southern Asia, bordering the Arabian Sea and the Bay of Bengal, between Burma and Pakistan. The capitol is New Delhi.	Republic of India
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012867	Skin	The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
D013019	South Africa	A republic in southern Africa, the southernmost part of Africa. It has three capitals: Pretoria (administrative), Cape Town (legislative), and Bloemfontein (judicial). Officially the Republic of South Africa since 1960, it was called the Union of South Africa 1910-1960.	Republic of South Africa,Union of South Africa
D013203	Staphylococcal Infections	Infections with bacteria of the genus STAPHYLOCOCCUS.	Infections, Staphylococcal,Staphylococcus aureus Infection,Staphylococcal Infection,Staphylococcus aureus Infections
D013211	Staphylococcus aureus	Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
D016106	Methicillin Resistance	Non-susceptibility of a microbe to the action of METHICILLIN, a semi-synthetic penicillin derivative.	Methicillin-Resistant,Methicillin Resistant,Resistance, Methicillin
D017720	Molecular Epidemiology	The application of molecular biology to the answering of epidemiological questions. The examination of patterns of changes in DNA to implicate particular carcinogens and the use of molecular markers to predict which individuals are at highest risk for a disease are common examples.	Epidemiology, Molecular,Genetic Epidemiology,Epidemiologies, Genetic,Epidemiologies, Molecular,Epidemiology, Genetic,Genetic Epidemiologies,Molecular Epidemiologies
D055624	Methicillin-Resistant Staphylococcus aureus	A strain of Staphylococcus aureus that is non-susceptible to the action of METHICILLIN. The mechanism of resistance usually involves modification of normal or the presence of acquired PENICILLIN BINDING PROTEINS.	MRSA,Methicillin Resistant Staphylococcus aureus