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Cell hybrids obtained by fusing mouse PCC4azal teratocarcinoma cells and spleen cells induced to proliferation and treated with the demethylating agent 5-azacytidine prior to fusion are described. The obtained hybrids demonstrated no expression of T lymphocyte marker genes CD11 and CD45, which indicates possible somatic nucleus reprogramming by factors present in teratocarcinoma cells. Irrespective of culture conditions, cell hybrids demonstrated a relatively stable chromosome number: they lost on average no more than four chromosomes after 30 passages. Culturing in medium containing hypoxanthine, aminopterin, and thymidine (selective conditions) decreased the differentiation capacity of cell hybrids compared to nonselective conditions, which is likely due to the inhibition of their metabolism. For the first time, teratocarcinoma cell hybrid differentiation into cardiomyocytes under the influence of DMSO has been demonstrated in vitro.
E I Filiasova, and
O V Zatsepina, and
Iu M Khodarovich, and
O A Larionov
March 1980,
Developmental biology,
E I Filiasova, and
O V Zatsepina, and
Iu M Khodarovich, and
O A Larionov
March 1980,
Developmental biology,
E I Filiasova, and
O V Zatsepina, and
Iu M Khodarovich, and
O A Larionov
March 1980,
Developmental biology,
E I Filiasova, and
O V Zatsepina, and
Iu M Khodarovich, and
O A Larionov
December 1983,
Molecular and cellular biology,
E I Filiasova, and
O V Zatsepina, and
Iu M Khodarovich, and
O A Larionov
January 1971,
Giornale di batteriologia, virologia, ed immunologia ed annali dell'Ospedale Maria Vittoria di Torino,
E I Filiasova, and
O V Zatsepina, and
Iu M Khodarovich, and
O A Larionov
January 2005,
Blood,
E I Filiasova, and
O V Zatsepina, and
Iu M Khodarovich, and
O A Larionov
January 1982,
Differentiation; research in biological diversity,
E I Filiasova, and
O V Zatsepina, and
Iu M Khodarovich, and
O A Larionov
October 1984,
Experimental cell research,
E I Filiasova, and
O V Zatsepina, and
Iu M Khodarovich, and
O A Larionov
October 1979,
Journal of cellular physiology,
E I Filiasova, and
O V Zatsepina, and
Iu M Khodarovich, and
O A Larionov
March 1977,
Experimental cell research,
