Exploring the activity of tobacco etch virus protease in detergent solutions. 2008

Anna-Karin Lundbäck, and Susanne van den Berg, and Hans Hebert, and Helena Berglund, and Said Eshaghi
Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.

Tobacco etch virus (TEV) protease is generally used to remove affinity tags from target proteins. It has been reported that some detergents inhibit the activity of this protease, and therefore should be avoided when removing affinity tags from membrane proteins. The aim of this study was to explore and evaluate this further. Hence, affinity tag removal with TEV protease was tested from three membrane proteins (a Pgp synthase and two CorA homologs) in the presence of 16 different detergents commonly used in membrane protein purification and crystallization. We observed that in the presence of the same detergent (Triton X-100), TEV protease could remove the affinity tag completely from one protein (CorA) but not from another protein (Pgp synthase). There was also a large variation in yield of cleaved membrane protein in different detergents, which probably depends on features of the protein-detergent complex. These observations show that, contrary to an earlier report, detergents do not inhibit the enzymatic activity of the TEV protease.

UI MeSH Term Description Entries
D010450 Endopeptidases A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS. Endopeptidase,Peptide Peptidohydrolases
D011480 Protease Inhibitors Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES). Antiprotease,Endopeptidase Inhibitor,Endopeptidase Inhibitors,Peptidase Inhibitor,Peptidase Inhibitors,Peptide Hydrolase Inhibitor,Peptide Hydrolase Inhibitors,Peptide Peptidohydrolase Inhibitor,Peptide Peptidohydrolase Inhibitors,Protease Antagonist,Protease Antagonists,Antiproteases,Protease Inhibitor,Antagonist, Protease,Antagonists, Protease,Hydrolase Inhibitor, Peptide,Hydrolase Inhibitors, Peptide,Inhibitor, Endopeptidase,Inhibitor, Peptidase,Inhibitor, Peptide Hydrolase,Inhibitor, Peptide Peptidohydrolase,Inhibitor, Protease,Inhibitors, Endopeptidase,Inhibitors, Peptidase,Inhibitors, Peptide Hydrolase,Inhibitors, Peptide Peptidohydrolase,Inhibitors, Protease,Peptidohydrolase Inhibitor, Peptide,Peptidohydrolase Inhibitors, Peptide
D003902 Detergents Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties. Cleansing Agents,Detergent Pods,Laundry Detergent Pods,Laundry Pods,Syndet,Synthetic Detergent,Agent, Cleansing,Agents, Cleansing,Cleansing Agent,Detergent,Detergent Pod,Detergent Pod, Laundry,Detergent Pods, Laundry,Detergent, Synthetic,Detergents, Synthetic,Laundry Detergent Pod,Laundry Pod,Pod, Detergent,Pod, Laundry,Pod, Laundry Detergent,Pods, Detergent,Pods, Laundry,Pods, Laundry Detergent,Synthetic Detergents
D012996 Solutions The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed) Solution
D017800 Potyvirus A large genus of plant viruses of the family POTYVIRIDAE which infect mainly plants of the Solanaceae. Transmission is primarily by aphids in a non-persistent manner. The type species is potato virus Y. Potato Virus Y,Potato Virus Ys,Potyviruses,Virus Ys, Potato,Ys, Potato Virus

Related Publications

Anna-Karin Lundbäck, and Susanne van den Berg, and Hans Hebert, and Helena Berglund, and Said Eshaghi
January 2003, Protein expression and purification,
Anna-Karin Lundbäck, and Susanne van den Berg, and Hans Hebert, and Helena Berglund, and Said Eshaghi
June 2002, Biochemical and biophysical research communications,
Anna-Karin Lundbäck, and Susanne van den Berg, and Hans Hebert, and Helena Berglund, and Said Eshaghi
October 2017, Protein engineering, design & selection : PEDS,
Anna-Karin Lundbäck, and Susanne van den Berg, and Hans Hebert, and Helena Berglund, and Said Eshaghi
August 2016, Journal of biotechnology,
Anna-Karin Lundbäck, and Susanne van den Berg, and Hans Hebert, and Helena Berglund, and Said Eshaghi
October 1987, Virology,
Anna-Karin Lundbäck, and Susanne van den Berg, and Hans Hebert, and Helena Berglund, and Said Eshaghi
April 2024, Proteins,
Anna-Karin Lundbäck, and Susanne van den Berg, and Hans Hebert, and Helena Berglund, and Said Eshaghi
November 2013, Protein expression and purification,
Anna-Karin Lundbäck, and Susanne van den Berg, and Hans Hebert, and Helena Berglund, and Said Eshaghi
December 2002, The Journal of biological chemistry,
Anna-Karin Lundbäck, and Susanne van den Berg, and Hans Hebert, and Helena Berglund, and Said Eshaghi
April 2013, Analytical biochemistry,
Anna-Karin Lundbäck, and Susanne van den Berg, and Hans Hebert, and Helena Berglund, and Said Eshaghi
March 2012, Protein expression and purification,
Copied contents to your clipboard!