BIOMAT Database Logo BIOMAT Database Logo

Elevated plasma platelet-derived growth factor B-chain levels in cancer patients. 1991

K Leitzel, and W Bryce, and J Tomita, and G Manderino, and I Tribby, and A Thomason, and M Billingsley, and E Podczaski, and H Harvey, and M Bartholomew
Department of Medicine, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033.

Platelet-derived growth factor (PDGF) is produced by a variety of normal and tumor cells in vitro. We have developed an enzyme-linked immunosorbent assay for the detection of the B-chain of PDGF. This assay can reliably detect 0.1 ng/ml of homodimeric recombinant PDGF B-chain and does not cross-react with recombinant PDGF-AA, epidermal growth factor, basic fibroblast growth factor, or transforming growth factor-beta. Citrated plasma from 72 control individuals had a PDGF B-chain (PDGF-B) level of 0.32 +/- 0.14 ng/ml (mean +/- SD) with a range of 0.10-0.69 ng/ml. The plasma platelet factor 4 (PF4) level was 97 +/- 70 ng/ml, with a range of 34-363 ng/ml. Citrated plasma was obtained from 131 cancer patients, and plasma PDGF-B was elevated in 19 (15%) of the patients. Both PDGF-B and PF4 were elevated in 14 (11%) of these patients, consistent with a platelet source of PDGF-B. In 5 patients (4%), however, PDGF-B was elevated and PF4 was not elevated compared to the control group. This last group of patients may have a tumor-derived source of PDGF-B which could be important in autocrine or paracrine growth stimulation of the tumor cells.

UI MeSH Term Description Entries
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D009367 Neoplasm Staging Methods which attempt to express in replicable terms the extent of the neoplasm in the patient. Cancer Staging,Staging, Neoplasm,Tumor Staging,TNM Classification,TNM Staging,TNM Staging System,Classification, TNM,Classifications, TNM,Staging System, TNM,Staging Systems, TNM,Staging, Cancer,Staging, TNM,Staging, Tumor,System, TNM Staging,Systems, TNM Staging,TNM Classifications,TNM Staging Systems
D009369 Neoplasms New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms. Benign Neoplasm,Cancer,Malignant Neoplasm,Tumor,Tumors,Benign Neoplasms,Malignancy,Malignant Neoplasms,Neoplasia,Neoplasm,Neoplasms, Benign,Cancers,Malignancies,Neoplasias,Neoplasm, Benign,Neoplasm, Malignant,Neoplasms, Malignant
D010982 Platelet-Derived Growth Factor Mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. Platelet Derived Growth Factor,Factor, Platelet-Derived Growth,Growth Factor, Platelet-Derived
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

K Leitzel, and W Bryce, and J Tomita, and G Manderino, and I Tribby, and A Thomason, and M Billingsley, and E Podczaski, and H Harvey, and M Bartholomew
Plasma platelet-derived growth factor-B chain is elevated in patients with extensively large brain tumour.
January 1995, Acta neurochirurgica,
K Leitzel, and W Bryce, and J Tomita, and G Manderino, and I Tribby, and A Thomason, and M Billingsley, and E Podczaski, and H Harvey, and M Bartholomew
Stromal expression of platelet-derived growth factor beta-receptor and platelet-derived growth factor B-chain in colorectal cancer.
December 1993, Laboratory investigation; a journal of technical methods and pathology,
K Leitzel, and W Bryce, and J Tomita, and G Manderino, and I Tribby, and A Thomason, and M Billingsley, and E Podczaski, and H Harvey, and M Bartholomew
sis (platelet-derived growth factor B chain) gene transcript levels are elevated in human atherosclerotic lesions compared to normal artery.
February 1987, Proceedings of the National Academy of Sciences of the United States of America,
K Leitzel, and W Bryce, and J Tomita, and G Manderino, and I Tribby, and A Thomason, and M Billingsley, and E Podczaski, and H Harvey, and M Bartholomew
Expression of platelet-derived growth factor B-chain and platelet-derived growth factor beta-receptor in fibroblasts of scleroderma.
November 1998, Journal of dermatological science,
K Leitzel, and W Bryce, and J Tomita, and G Manderino, and I Tribby, and A Thomason, and M Billingsley, and E Podczaski, and H Harvey, and M Bartholomew
Inhibitory DNA ligands to platelet-derived growth factor B-chain.
November 1996, Biochemistry,
K Leitzel, and W Bryce, and J Tomita, and G Manderino, and I Tribby, and A Thomason, and M Billingsley, and E Podczaski, and H Harvey, and M Bartholomew
Elevated plasma levels of platelet-activating factor (PAF) in breast cancer patients with hypercalcemia.
January 1989, Journal of lipid mediators,
K Leitzel, and W Bryce, and J Tomita, and G Manderino, and I Tribby, and A Thomason, and M Billingsley, and E Podczaski, and H Harvey, and M Bartholomew
Regulation of the human platelet-derived growth factor B-chain gene.
December 1993, Hiroshima journal of medical sciences,
K Leitzel, and W Bryce, and J Tomita, and G Manderino, and I Tribby, and A Thomason, and M Billingsley, and E Podczaski, and H Harvey, and M Bartholomew
Platelet-derived growth factor B-chain-like immunoreactivity in injured rat brain.
August 1994, Brain research,
K Leitzel, and W Bryce, and J Tomita, and G Manderino, and I Tribby, and A Thomason, and M Billingsley, and E Podczaski, and H Harvey, and M Bartholomew
Assignment of intrachain disulfide bonds in platelet-derived growth factor B-chain.
June 1993, The Journal of biological chemistry,
K Leitzel, and W Bryce, and J Tomita, and G Manderino, and I Tribby, and A Thomason, and M Billingsley, and E Podczaski, and H Harvey, and M Bartholomew
Plasma levels of platelet-derived growth factor BB and transforming growth factor in patients with failed hip prostheses.
February 2005, Acta orthopaedica,
Copied contents to your clipboard!