Biomaterial Database

Fluoroquinolone resistance in clinical isolates of Klebsiella oxytoca. 2008

Toshinobu Horii, and Mai Osaki, and Hideaki Muramatsu

Division of Bacteriology, Department of Microbiology and Immunology, Tottori University Faculty of Medicine, Yonago, Japan. horii@med.tottori-u.ac.jp

BACKGROUND Prevalence of fluoroquinolone-resistant Klebsiella oxytoca has been reported worldwide. METHODS We recovered ten clinical K. oxytoca isolates from patients with acute cystitis, asymptomatic bacteriuria or acute bacillary diarrhea in Japan. Out of ten isolates, one fluoroquinolone-susceptible isolate was included as a control. Fluoroquinolone resistance was characterized genetically by PCR and DNA sequencing methods. Outer membrane protein (OMP) profiles were determined by SDS-PAGE. RESULTS In nine clinical isolates of levofloxacin-resistant K. oxytoca, nucleotide sequences in the quinolone-resistance-determining regions showed amino acid mutations such as Thr83Ile and Asp87Gly in GyrA and Ser80Ile in ParC. Combined effects of reduced 36-kDa OMP production and amino acid mutations in GyrA and ParC were shown by two K. oxytoca isolates exhibiting higher minimum inhibitory concentrations for fluoroquinolones than other fluoroquinolone-resistant isolates. CONCLUSIONS In clinical K. oxytoca isolates, the various mechanisms of fluoroquinolone resistance may include reduced 36-kDa OMP production as well as GyrA and ParC mutations.

UI	MeSH Term	Description	Entries
D007710	Klebsiella Infections	Infections with bacteria of the genus KLEBSIELLA.	Infections, Klebsiella,Infection, Klebsiella,Klebsiella Infection
D004198	Disease Susceptibility	A constitution or condition of the body which makes the tissues react in special ways to certain extrinsic stimuli and thus tends to make the individual more than usually susceptible to certain diseases.	Diathesis,Susceptibility, Disease,Diatheses,Disease Susceptibilities,Susceptibilities, Disease
D005243	Feces	Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000900	Anti-Bacterial Agents	Substances that inhibit the growth or reproduction of BACTERIA.	Anti-Bacterial Agent,Anti-Bacterial Compound,Anti-Mycobacterial Agent,Antibacterial Agent,Antibiotics,Antimycobacterial Agent,Bacteriocidal Agent,Bacteriocide,Anti-Bacterial Compounds,Anti-Mycobacterial Agents,Antibacterial Agents,Antibiotic,Antimycobacterial Agents,Bacteriocidal Agents,Bacteriocides,Agent, Anti-Bacterial,Agent, Anti-Mycobacterial,Agent, Antibacterial,Agent, Antimycobacterial,Agent, Bacteriocidal,Agents, Anti-Bacterial,Agents, Anti-Mycobacterial,Agents, Antibacterial,Agents, Antimycobacterial,Agents, Bacteriocidal,Anti Bacterial Agent,Anti Bacterial Agents,Anti Bacterial Compound,Anti Bacterial Compounds,Anti Mycobacterial Agent,Anti Mycobacterial Agents,Compound, Anti-Bacterial,Compounds, Anti-Bacterial
D024841	Fluoroquinolones	A group of QUINOLONES with at least one fluorine atom and a piperazinyl group.	Fluoroquinolone
D024881	Drug Resistance, Bacterial	The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).	Antibiotic Resistance, Bacterial,Antibacterial Drug Resistance
D027081	DNA Gyrase	A bacterial DNA topoisomerase II that catalyzes ATP-dependent breakage of both strands of DNA, passage of the unbroken strands through the breaks, and rejoining of the broken strands. Gyrase binds to DNA as a heterotetramer consisting of two A and two B subunits. In the presence of ATP, gyrase is able to convert the relaxed circular DNA duplex into a superhelix. In the absence of ATP, supercoiled DNA is relaxed by DNA gyrase.	DNA Gyrase A Subunit,DNA Gyrase B Subunit,DNA-Gyrase,GyrA Protein,GyrB Protein
D027101	DNA Topoisomerase IV	A bacterial DNA topoisomerase II that catalyzes ATP-dependent breakage of both strands of DNA, passage of the unbroken strands through the breaks, and rejoining of the broken strands. Topoisomerase IV binds to DNA as a heterotetramer consisting 2 parC and 2 parE subunits. Topoisomerase IV is a decatenating enzyme that resolves interlinked daughter chromosomes following DNA replication.	parC Protein,parE Protein,Topo IV,Topoisomerase IV,Topoisomerase IV Subunit A,Topoisomerase IV Subunit B,parC Gene Product,parC Gene Product, Topo IV,parE Gene Product,parE Gene Product, Topo IV,Topoisomerase IV, DNA
D041121	Klebsiella oxytoca	A species of gram-negative bacteria causing URINARY TRACT INFECTIONS and SEPTICEMIA.	Bacillus oxytocus perniciosus