The aim of this study was to assess the neuropsychomotor development of children enrolled in daycare centers and preschools. This cross-sectional study used 38 items from the Denver II test to assess four and five-year-olds enrolled in the municipal school system in Cuiabá, Mato Grosso State, Brazil, from August 2002 to November 2003. There were 960 children enrolled in 27 daycare centers and two public preschools. Statistical analysis used the chi2 test with a 95% confidence interval and alpha = 5%. Logistic regression was used to calculate the percentages with which the preschoolers passed the test at each respective age. Of the 960 preschoolers tested, 67% showed normal performance, 30.2% borderline, and 2.8% abnormal. In 27 of the 38 items, the proportion of correct answers was greater than 90%. Altered performance was more common in five-year-old boys. Performance in this sample was quite similar to that of preschoolers in Denver, Colorado, USA. The best results by gender were for girls, and by age in four-year-olds.