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Organ cultures of central and peripheral human corneal epithelium were maintained for 1 and 2 weeks in Hams F10 medium supplemented with 20% foetal bovine serum, and in medium conditioned by conjunctival epithelial cells. Light microscopy and scanning electron microscopy showed no obvious differences in morphology and survival between central and peripheral explants, or between explants cultured in conditioned and non-conditioned medium. Live cells and areas with confluent growth were seen in all types of explants. The results demonstrate that differences between growth of central and peripheral corneal epithelium in vitro do not necessarily reflect different proliferative potential in vivo. The previously reported lack of survival of central corneal epithelium in culture may reflect an insufficient in vitro system.
K U Sandvig, and
B Nicolaissen, and
E Haaskjold
January 2011,
Klinika oczna,
K U Sandvig, and
B Nicolaissen, and
E Haaskjold
January 1981,
In vitro,
K U Sandvig, and
B Nicolaissen, and
E Haaskjold
November 1989,
Investigative ophthalmology & visual science,
K U Sandvig, and
B Nicolaissen, and
E Haaskjold
December 1993,
Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft,
K U Sandvig, and
B Nicolaissen, and
E Haaskjold
September 1988,
Archives of ophthalmology (Chicago, Ill. : 1960),
K U Sandvig, and
B Nicolaissen, and
E Haaskjold
March 1996,
Investigative ophthalmology & visual science,
K U Sandvig, and
B Nicolaissen, and
E Haaskjold
January 1990,
Bulletin des societes d'ophtalmologie de France,
K U Sandvig, and
B Nicolaissen, and
E Haaskjold
February 1993,
Acta ophthalmologica,
K U Sandvig, and
B Nicolaissen, and
E Haaskjold
January 1991,
Journal francais d'ophtalmologie,
K U Sandvig, and
B Nicolaissen, and
E Haaskjold
September 1995,
Cornea,
