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Five distinctly different types of naphthyl acetate esterase in rat liver were examined for study of liver enzymes during hepatocarcinogenesis. Three types of esterase in normal adult liver were separated by column chromatography. Main esterase in adult hepatocytes, which was demonstrated near the origin in cellulose acetate electrophoresis, was very sensitive to diisopropyl phosphorofluoridate (DFP). The other two esterases showed different electrophoretic mobility, while their Km values did not differ and both were considerably resistant to DFP. An anodic minor component in normal adult liver, which had a characteristic esterase pattern of infant liver, increased in the liver of rats fed 3'-methyl-4-(dimethylamino) azobenzene. This esterase was obtained by electrophoresis on Cellogel block. It was partially inhibited by DFP and p-chloromercuribenzoate, activated by cysteine, and showed a different Km value from the above esterases. Another minor component situated at the most cathodic side, which had characteristic esterase patterns of fetal liver and hepatoma, was very sensitive to DFP and eserine, and showed a characteristic of nonspecific cholinesterase as proved by staining.
A Kaneko, and
Y Yoshida, and
S Yokoyama, and
K Dempo, and
T Onoé
February 1980,
Gan,
A Kaneko, and
Y Yoshida, and
S Yokoyama, and
K Dempo, and
T Onoé
December 1980,
Gan,
A Kaneko, and
Y Yoshida, and
S Yokoyama, and
K Dempo, and
T Onoé
April 1974,
Gan,
A Kaneko, and
Y Yoshida, and
S Yokoyama, and
K Dempo, and
T Onoé
February 1982,
Cancer letters,
A Kaneko, and
Y Yoshida, and
S Yokoyama, and
K Dempo, and
T Onoé
April 1982,
Japanese journal of pharmacology,
A Kaneko, and
Y Yoshida, and
S Yokoyama, and
K Dempo, and
T Onoé
October 1975,
Gan,
A Kaneko, and
Y Yoshida, and
S Yokoyama, and
K Dempo, and
T Onoé
September 1972,
International journal of cancer,
A Kaneko, and
Y Yoshida, and
S Yokoyama, and
K Dempo, and
T Onoé
May 1989,
Chemical & pharmaceutical bulletin,
A Kaneko, and
Y Yoshida, and
S Yokoyama, and
K Dempo, and
T Onoé
October 1983,
Journal of the National Cancer Institute,
A Kaneko, and
Y Yoshida, and
S Yokoyama, and
K Dempo, and
T Onoé
June 1963,
Experientia,
