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Proposal for a procedure for complete inactivation of the Creutzfeldt-Jakob disease agent. 1991
F Taguchi, and
Y Tamai, and
K Uchida, and
R Kitajima, and
H Kojima, and
T Kawaguchi, and
Y Ohtani, and
S Miura
Department of Microbiology, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
We have examined complete inactivation conditions on brain homogenates from mice affected with Creutzfeldt-Jakob disease agent, and recommend for routine use a reliable procedure first treating the affected materials with 1 N NaOH for 60 min and then autoclaving at 121 degrees C for 30 min.
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