-transparent.png){kind=logo}
Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems. 2008
Beatriz Maestro, and
Isabel Velasco, and
Isabel Castillejo, and
Miguel Arévalo-Rodríguez, and
Angel Cebolla, and
Jesús M Sanz
Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Avda. Universidad s/n, 03202 Elche (Alicante), Spain.
We present a novel procedure for affinity partitioning of recombinant proteins fused to the choline-binding module C-LytA in aqueous two-phase systems containing poly(ethylene glycol) (PEG). Proteins tagged with the C-LytA module and exposed to the two-phase systems are quantitatively localized in the PEG-rich phase, whereas subsequent addition of the natural ligand choline specifically shifts their localization to the PEG-poor phase by displacement of the polymer from the binding sites. The described procedure is simple, scalable and reproducible, and has been successfully applied to the purification of four diverse proteins, resulting in high yields and purity.
Related Publications
Beatriz Maestro, and
Isabel Velasco, and
Isabel Castillejo, and
Miguel Arévalo-Rodríguez, and
Angel Cebolla, and
Jesús M Sanz
January 2004,
Biotechnology progress,
Beatriz Maestro, and
Isabel Velasco, and
Isabel Castillejo, and
Miguel Arévalo-Rodríguez, and
Angel Cebolla, and
Jesús M Sanz
August 2007,
Journal of chromatography. A,
Beatriz Maestro, and
Isabel Velasco, and
Isabel Castillejo, and
Miguel Arévalo-Rodríguez, and
Angel Cebolla, and
Jesús M Sanz
June 2012,
Journal of chromatography. A,
Beatriz Maestro, and
Isabel Velasco, and
Isabel Castillejo, and
Miguel Arévalo-Rodríguez, and
Angel Cebolla, and
Jesús M Sanz
December 2002,
Biochimica et biophysica acta,
Beatriz Maestro, and
Isabel Velasco, and
Isabel Castillejo, and
Miguel Arévalo-Rodríguez, and
Angel Cebolla, and
Jesús M Sanz
February 1991,
Journal of chromatography,
Beatriz Maestro, and
Isabel Velasco, and
Isabel Castillejo, and
Miguel Arévalo-Rodríguez, and
Angel Cebolla, and
Jesús M Sanz
January 1995,
Bioseparation,
Beatriz Maestro, and
Isabel Velasco, and
Isabel Castillejo, and
Miguel Arévalo-Rodríguez, and
Angel Cebolla, and
Jesús M Sanz
February 1975,
The Journal of biological chemistry,
Beatriz Maestro, and
Isabel Velasco, and
Isabel Castillejo, and
Miguel Arévalo-Rodríguez, and
Angel Cebolla, and
Jesús M Sanz
February 1995,
Bioseparation,
Beatriz Maestro, and
Isabel Velasco, and
Isabel Castillejo, and
Miguel Arévalo-Rodríguez, and
Angel Cebolla, and
Jesús M Sanz
October 2007,
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Beatriz Maestro, and
Isabel Velasco, and
Isabel Castillejo, and
Miguel Arévalo-Rodríguez, and
Angel Cebolla, and
Jesús M Sanz
February 2017,
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
