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Pitx1 expression in developing and regenerating axolotl limbs. 2008

Takashi Shimokawa, and Satoru Yasutaka, and Rieko Kominami, and Shigenori Tanaka, and Harumichi Shinohara
Division of Anatomy II, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, 920-0293 Japan. simoort1@kanazawa-med.ac.jp

The expression of the homeobox transcription factor Pitx1 was investigated in the Mexican axolotl (Ambystoma mexicanum) during limb development and regeneration by whole-mount mRNA in situ hybridizations. This clone shares high amino acid identity with Pitx1 from other vertebrates (92% Xenopus; 87% chick; 75% human and mouse) within the region isolated. In the developing limbs, Pitx1 was expressed in hindlimb mesenchyme, as has been reported in other species. The expression pattern in the hindlimb might have been conserved during evolution. In the regenerating limbs, Pitx1 was expressed in both fore- and hindlimb blastemas. Our observations suggest two roles of Pitx1 in the axolotl: one is to determine the hindlimb pattern during development, and the other that relates to proliferation of regenerating tissues without regard to fore- or hindlimb.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009024 Morphogenesis The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
D012038 Regeneration The physiological renewal, repair, or replacement of tissue. Endogenous Regeneration,Regeneration, Endogenous,Regenerations
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D005121 Extremities The farthest or outermost projections of the body, such as the HAND and FOOT. Limbs,Extremity,Limb
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000558 Ambystoma mexicanum A salamander found in Mexican mountain lakes and accounting for about 30 percent of the urodeles used in research. The axolotl remains in larval form throughout its life, a phenomenon known as neoteny. Axolotl,Mexican Salamander,Ambystoma mexicanums,Axolotls,Salamander, Mexican,mexicanums, Ambystoma
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions

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