Biomaterial Database

[De-endothelialization and re-endothelialization in autogenous vein grafts--fundamental and clinical study]. 1991

M Horio

First Department of Surgery, Asahikawa Medical College, Japan.

Preparation for preserving endothelial cells (ECs) of autogenous vein grafts (AVGs) and the process of de-endothelialization (de-E) and re-endothelialization (re-E) were studied. Jugular veins (n = 31) from mongrel dogs were immersed in saline solution (SS), heparinized blood (HB), and other three kinds of solution. Then jugular veins (n = 26) immersed in SS for 3 hours, were used for AVGs to the abdominal aorta. Intimal changes were observed by scanning electron microscopy. ECs showed irreversible changes about 60 minutes after immersion in SS and about 150 minutes in HB. Eleven (92%) of 12 graft specimens within 1 day after implantation showed wide areas of de-E, while ECs on the venous valve were satisfactorily preserved in 6 (55%) of 11 valves. Re-E was observed predominantly around the valve from 3 days after implantation, and completed within 3 weeks. Clinically, specimens in 35 AVGs were collected before anastomosis. Severe EC damage was observed in 23 (66%) of them. Long-term observation revealed graft stenosis in 2 due to intimal hyperplasia (IH) in grafts with severely damaged EC. These results suggest that venous valves are an important source of re-E, and that the preservation of ECs in graft preparation is an important factor to prevent IH.

UI	MeSH Term	Description	Entries
D012038	Regeneration	The physiological renewal, repair, or replacement of tissue.	Endogenous Regeneration,Regeneration, Endogenous,Regenerations
D004285	Dogs	The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)	Canis familiaris,Dog
D004730	Endothelium, Vascular	Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.	Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D006083	Graft Occlusion, Vascular	Obstruction of flow in biological or prosthetic vascular grafts.	Graft Restenosis, Vascular,Vascular Graft Occlusion,Vascular Graft Restenosis,Graft Restenoses, Vascular,Occlusion, Vascular Graft,Restenosis, Vascular Graft
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014022	Tissue Survival	The span of viability of a tissue or an organ.	Organ Survival,Organ Viability,Tissue Viability,Survival, Organ,Survival, Tissue,Viability, Organ,Viability, Tissue
D014182	Transplantation, Autologous	Transplantation of an individual's own tissue from one site to another site.	Autografting,Autologous Transplantation,Autotransplantation,Autograftings,Autologous Transplantations,Autotransplantations,Transplantations, Autologous
D014680	Veins	The vessels carrying blood away from the CAPILLARY BEDS.	Vein