Histamine H1-receptor-mediated keratan sulfate production in rabbit chondrocytes: involvement of protein kinase C. 1991

K Fukuda, and H Yamasaki, and Y Nagata, and H Motoyoshi, and F Matsumura, and T Kuno, and S Tanaka
Department of Orthopedic Surgery, Kinki University School of Medicine, Osaka, Japan.

We investigated the characteristics of the histamine H1-receptor in cultured rabbit chondrocytes. Scatchard analysis of [3H]pyrilamine, an H1-antagonist, binding to the chondrocytes revealed a single class of binding sites with KD and Bmax values of 90 +/- 12 nM and 56 +/- 11 fmol/10(4) cells, respectively. H1-agonists stimulated the production of keratan sulfate in a dose-dependent manner. Stimulation of keratan sulfate production was inhibited by pyrilamine. Protein kinase C inhibitors (sphingosine and H-7) also had inhibitory effects. Phorbol 12,13-dibutyrate, a direct activator of protein kinase C, activated the production. When protein kinase C in the chondrocytes was down-regulated by preincubation with phorbol ester, the effect of the H1-agonist on keratan sulfate production was abolished. These results indicate that the histamine H1-receptor on chondrocytes mediates the accumulation of keratan sulfate production and that protein kinase C is involved in these events.

UI MeSH Term Description Entries
D007632 Keratan Sulfate A sulfated mucopolysaccharide initially isolated from bovine cornea. At least two types are known. Type I, found mostly in the cornea, contains D-galactose and D-glucosamine-6-O-sulfate as the repeating unit; type II, found in skeletal tissues, contains D-galactose and D-galactosamine-6-O-sulfate as the repeating unit. Keratosulfate,Sulfate, Keratan
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008761 Methylhistamines Histamine substituted in any position with one or more methyl groups. Many of these are agonists for the H1, H2, or both histamine receptors.
D011493 Protein Kinase C An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters. Calcium Phospholipid-Dependent Protein Kinase,Calcium-Activated Phospholipid-Dependent Kinase,PKC Serine-Threonine Kinase,Phospholipid-Sensitive Calcium-Dependent Protein Kinase,Protein Kinase M,Calcium Activated Phospholipid Dependent Kinase,Calcium Phospholipid Dependent Protein Kinase,PKC Serine Threonine Kinase,Phospholipid Sensitive Calcium Dependent Protein Kinase,Phospholipid-Dependent Kinase, Calcium-Activated,Serine-Threonine Kinase, PKC
D011738 Pyrilamine A histamine H1 antagonist. It has mild hypnotic properties and some local anesthetic action and is used for allergies (including skin eruptions) both parenterally and locally. It is a common ingredient of cold remedies. Mepyramine,Pyranisamine,Anthisan,Boots Bite & Sting Relief,Kriptin,Mepyramine Maleate,Pyrilamine Maleate,Maleate, Mepyramine,Maleate, Pyrilamine
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D011969 Receptors, Histamine H1 A class of histamine receptors discriminated by their pharmacology and mode of action. Most histamine H1 receptors operate through the inositol phosphate/diacylglycerol second messenger system. Among the many responses mediated by these receptors are smooth muscle contraction, increased vascular permeability, hormone release, and cerebral glyconeogenesis. (From Biochem Soc Trans 1992 Feb;20(1):122-5) H1 Receptor,Histamine H1 Receptors,H1 Receptors,Histamine H1 Receptor,Receptors, H1,H1 Receptor, Histamine,H1 Receptors, Histamine,Receptor, H1,Receptor, Histamine H1
D002358 Cartilage, Articular A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact. Articular Cartilage,Articular Cartilages,Cartilages, Articular
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002744 Chlorpheniramine A histamine H1 antagonist used in allergic reactions, hay fever, rhinitis, urticaria, and asthma. It has also been used in veterinary applications. One of the most widely used of the classical antihistaminics, it generally causes less drowsiness and sedation than PROMETHAZINE. Chlorphenamine,Chlorprophenpyridamine,Aller-Chlor,Antihistaminico Llorens,Chlo-Amine,Chlor-100,Chlor-Trimeton,Chlor-Tripolon,Chlorpheniramine Maleate,Chlorpheniramine Tannate,Chlorpro,Chlorspan 12,Chlortab-4,Cloro-Trimeton,Efidac 24,Kloromin,Piriton,Teldrin,Maleate, Chlorpheniramine,Tannate, Chlorpheniramine

Related Publications

K Fukuda, and H Yamasaki, and Y Nagata, and H Motoyoshi, and F Matsumura, and T Kuno, and S Tanaka
December 1993, The American journal of physiology,
K Fukuda, and H Yamasaki, and Y Nagata, and H Motoyoshi, and F Matsumura, and T Kuno, and S Tanaka
April 1989, Agents and actions,
K Fukuda, and H Yamasaki, and Y Nagata, and H Motoyoshi, and F Matsumura, and T Kuno, and S Tanaka
January 1991, Agents and actions. Supplements,
K Fukuda, and H Yamasaki, and Y Nagata, and H Motoyoshi, and F Matsumura, and T Kuno, and S Tanaka
April 1995, Inflammation research : official journal of the European Histamine Research Society ... [et al.],
K Fukuda, and H Yamasaki, and Y Nagata, and H Motoyoshi, and F Matsumura, and T Kuno, and S Tanaka
April 2005, Inflammation research : official journal of the European Histamine Research Society ... [et al.],
K Fukuda, and H Yamasaki, and Y Nagata, and H Motoyoshi, and F Matsumura, and T Kuno, and S Tanaka
November 1993, Biochemical Society transactions,
K Fukuda, and H Yamasaki, and Y Nagata, and H Motoyoshi, and F Matsumura, and T Kuno, and S Tanaka
July 1995, Journal of neurochemistry,
K Fukuda, and H Yamasaki, and Y Nagata, and H Motoyoshi, and F Matsumura, and T Kuno, and S Tanaka
August 1989, The Journal of biological chemistry,
K Fukuda, and H Yamasaki, and Y Nagata, and H Motoyoshi, and F Matsumura, and T Kuno, and S Tanaka
October 1998, Nihon yakurigaku zasshi. Folia pharmacologica Japonica,
K Fukuda, and H Yamasaki, and Y Nagata, and H Motoyoshi, and F Matsumura, and T Kuno, and S Tanaka
October 1992, British journal of pharmacology,
Copied contents to your clipboard!