Biomaterial Database

Cellular mechanisms of Cl- transport in outer medullary collecting duct. 1991

B Koeppen, and C Pappas, and T Manger, and A Oyler

Division of Nephrology, University of Connecticut Health Center, Farmington.

The OMCDi secretes Cl- when perfused and bathed with symmetrical solutions. The route for this transepithelial Cl- movement appears to be the paracellular pathway, and the driving force is the lumen positive voltage generated by the process of electrogenic H+ secretion. Currently, there is no evidence to support the existence of a significant transcellular route for transepithelial Cl- movement. Although the primary function of the OMCDi is related to urine acidification, Cl- plays an important role in this process. The basolateral membrane of the OMCDi cell contains a band-3 related Cl-/HCO3- antiporter and a Cl- conductance. The Cl-/HCO3- antiporter serves as the primary route for the efflux of HCO3- generated during the process of H+ secretion. The Cl- conductance allows Cl- brought into the cell by the antiporter to recycle across this membrane. This conductance also serves to maintain cell charge balance. Accordingly, for each equivalent of H+ leaving the cell across the apical membrane, a Cl- equivalent exits the cell across the basolateral membrane. Based on whole-cell patch-clamp studies, this Cl- conductance is blocked by the Cl- channel blocker DPC. This conductance may also have a finite permeability to HCO3-, and thus could serve as a secondary route for cellular HCO3- efflux. Lastly, the conductance is activated by the beta-adrenergic agonist isoproterenol.

UI	MeSH Term	Description	Entries
D007679	Kidney Medulla	The internal portion of the kidney, consisting of striated conical masses, the renal pyramids, whose bases are adjacent to the cortex and whose apices form prominent papillae projecting into the lumen of the minor calyces.	Kidney Papilla,Kidney Medullas,Kidney Papillas,Medulla, Kidney,Medullas, Kidney,Papilla, Kidney,Papillas, Kidney
D007685	Kidney Tubules, Collecting	Straight tubes commencing in the radiate part of the kidney cortex where they receive the curved ends of the distal convoluted tubules. In the medulla the collecting tubules of each pyramid converge to join a central tube (duct of Bellini) which opens on the summit of the papilla.	Kidney Collecting Ducts,Kidney Collecting Duct,Collecting Duct, Kidney,Collecting Ducts, Kidney
D008565	Membrane Proteins	Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.	Cell Membrane Protein,Cell Membrane Proteins,Cell Surface Protein,Cell Surface Proteins,Integral Membrane Proteins,Membrane-Associated Protein,Surface Protein,Surface Proteins,Integral Membrane Protein,Membrane Protein,Membrane-Associated Proteins,Membrane Associated Protein,Membrane Associated Proteins,Membrane Protein, Cell,Membrane Protein, Integral,Membrane Proteins, Integral,Protein, Cell Membrane,Protein, Cell Surface,Protein, Integral Membrane,Protein, Membrane,Protein, Membrane-Associated,Protein, Surface,Proteins, Cell Membrane,Proteins, Cell Surface,Proteins, Integral Membrane,Proteins, Membrane,Proteins, Membrane-Associated,Proteins, Surface,Surface Protein, Cell
D002352	Carrier Proteins	Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes.	Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D002712	Chlorides	Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.	Chloride,Chloride Ion Level,Ion Level, Chloride,Level, Chloride Ion
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001692	Biological Transport	The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.	Transport, Biological,Biologic Transport,Transport, Biologic
D027963	Chloride-Bicarbonate Antiporters	Electroneutral chloride bicarbonate exchangers that allow the exchange of BICARBONATE IONS exchange for CHLORIDE IONS across the cellular membrane. The action of specific antiporters in this class serve important functions such as allowing the efficient exchange of bicarbonate across red blood cell membranes as they passage through capillaries and the reabsorption of bicarbonate ions by the kidney.	AE Proteins,AE2 Anion Exchanger,AE2 Chloride-Bicarbonate Exchanger,AE3 Anion Exchanger,AE4 Anion Exchanger,Anion Exchange Protein 2,Anion Exchange Protein 3,Anion Exchange Protein 4,Anion Exchanger 2,Anion Exchanger 3,Anion Exchanger 4,Bicarbonate-Chloride Antiport,Cardiac Brain Band 3-Like Protein,Chloride-Bicarbonate Exchanger,Chloride-Bicarbonate Exchangers,Cl(-)-HCO3(-)-Exchanger,Cl- HCO3- Exchanger,Cl- HCO3- Exchangers,Neuronal Band 3-Like Protein,Non-Erythroid Band 3-Like Protein,SLC4A2 Protein,SLC4A3 Protein,SLC4A9 Protein,Sodium Bicarbonate Cotransporter 5,Solute Carrier Family 4 Member 2,Solute Carrier Family 4 Member 3,Solute Carrier Family 4 Member 9,Solute Carrier Family 4, Sodium Bicarbonate Cotransporter, Member 9,AE2 Chloride Bicarbonate Exchanger,Anion Exchanger, AE2,Anion Exchanger, AE3,Anion Exchanger, AE4,Antiport, Bicarbonate-Chloride,Antiporters, Chloride-Bicarbonate,Bicarbonate Chloride Antiport,Cardiac Brain Band 3 Like Protein,Chloride Bicarbonate Antiporters,Chloride Bicarbonate Exchanger,Chloride Bicarbonate Exchangers,Chloride-Bicarbonate Exchanger, AE2,Cl HCO3 Exchanger,Cl HCO3 Exchangers,Exchanger, Chloride-Bicarbonate,Exchangers, Chloride-Bicarbonate,Exchangers, Cl- HCO3-,HCO3- Exchangers, Cl-,Neuronal Band 3 Like Protein,Non Erythroid Band 3 Like Protein,Protein, SLC4A2,Protein, SLC4A3,Protein, SLC4A9,Proteins, AE