Hepatic gene expression changes in an experimental model of accelerated senescence: the SAM-P8 mouse. 2008

Laia Vilà, and Núria Roglans, and Marta Alegret, and Antoni Camins, and Mercè Pallàs, and Rosa María Sánchez, and Manuel Vázquez-Carrera, and Juan Carlos Laguna
Pharmacology Unit, Department of Pharmacology and Therapeutic Chemistry, School of Pharmacy, University of Barcelona, Spain.

The senescence-accelerated mouse (SAM) is an experimental model of aging, established through phenotypic selection from a common genetic pool of AKR/J mice. Here we use complementary DNA microarray, Western blot, and electrophoretic mobility shift assay to consider whether changes in liver gene expression observed in 5-month-old SAM-prone 8 (P8) mice, compared to SAM-R1 controls, are similar to those reported in aged rodents. Livers from SAM-P8 mice presented 88 differentially expressed transcripts, 59% of which were upregulated and 41% were downregulated. Of these, 14% were related to inflammatory/immunity processes, 10% were related to the xenobiotic metabolism (XM) and 3% to nervous system pathophysiology (NSP). Depressed expression and activity of genes related to XM, and altered expression of genes related to NSP, are similar to changes observed in aged rodents. Increased expression of heat shock protein 1 and Jun-B, reduced activity of activator protein 1 and absence of nuclear factor-kappaB activation indicate the lack of a strong liver inflammatory response in 5-month-old SAM-P8 mice.

UI MeSH Term Description Entries
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008297 Male Males
D008806 Mice, Inbred AKR An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. Mice, AKR,Mouse, AKR,Mouse, Inbred AKR,AKR Mice,AKR Mice, Inbred,AKR Mouse,AKR Mouse, Inbred,Inbred AKR Mice,Inbred AKR Mouse
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D000375 Aging The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time. Senescence,Aging, Biological,Biological Aging
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D018709 Statistics, Nonparametric A class of statistical methods applicable to a large set of probability distributions used to test for correlation, location, independence, etc. In most nonparametric statistical tests, the original scores or observations are replaced by another variable containing less information. An important class of nonparametric tests employs the ordinal properties of the data. Another class of tests uses information about whether an observation is above or below some fixed value such as the median, and a third class is based on the frequency of the occurrence of runs in the data. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1284; Corsini, Concise Encyclopedia of Psychology, 1987, p764-5) Kolmogorov-Smirnov Test,Kruskal-Wallis H Statistic,Mann-Whitney U Test,Rank-Sum Tests,Spearman Rank Correlation Coefficient,Wilcox Test,Wilcoxon Rank Test,Non-Parametric Statistics,Nonparametric Statistics,Statistics, Non-Parametric,Kolmogorov Smirnov Test,Mann Whitney U Test,Non Parametric Statistics,Rank Sum Tests,Rank Test, Wilcoxon,Rank-Sum Test,Statistics, Non Parametric,Test, Kolmogorov-Smirnov,Test, Mann-Whitney U,Test, Rank-Sum,Test, Wilcox,Test, Wilcoxon Rank,Tests, Rank-Sum,U Test, Mann-Whitney
D020133 Reverse Transcriptase Polymerase Chain Reaction A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols. Polymerase Chain Reaction, Reverse Transcriptase,Reverse Transcriptase PCR,PCR, Reverse Transcriptase,Transcriptase PCR, Reverse

Related Publications

Laia Vilà, and Núria Roglans, and Marta Alegret, and Antoni Camins, and Mercè Pallàs, and Rosa María Sánchez, and Manuel Vázquez-Carrera, and Juan Carlos Laguna
April 1998, Physiology & behavior,
Laia Vilà, and Núria Roglans, and Marta Alegret, and Antoni Camins, and Mercè Pallàs, and Rosa María Sánchez, and Manuel Vázquez-Carrera, and Juan Carlos Laguna
January 1997, Journal of neural transmission (Vienna, Austria : 1996),
Laia Vilà, and Núria Roglans, and Marta Alegret, and Antoni Camins, and Mercè Pallàs, and Rosa María Sánchez, and Manuel Vázquez-Carrera, and Juan Carlos Laguna
September 1991, Journal of the American Geriatrics Society,
Laia Vilà, and Núria Roglans, and Marta Alegret, and Antoni Camins, and Mercè Pallàs, and Rosa María Sánchez, and Manuel Vázquez-Carrera, and Juan Carlos Laguna
August 2005, Biological & pharmaceutical bulletin,
Laia Vilà, and Núria Roglans, and Marta Alegret, and Antoni Camins, and Mercè Pallàs, and Rosa María Sánchez, and Manuel Vázquez-Carrera, and Juan Carlos Laguna
December 2007, Biogerontology,
Laia Vilà, and Núria Roglans, and Marta Alegret, and Antoni Camins, and Mercè Pallàs, and Rosa María Sánchez, and Manuel Vázquez-Carrera, and Juan Carlos Laguna
January 1997, Experimental gerontology,
Laia Vilà, and Núria Roglans, and Marta Alegret, and Antoni Camins, and Mercè Pallàs, and Rosa María Sánchez, and Manuel Vázquez-Carrera, and Juan Carlos Laguna
April 2007, Brain research,
Laia Vilà, and Núria Roglans, and Marta Alegret, and Antoni Camins, and Mercè Pallàs, and Rosa María Sánchez, and Manuel Vázquez-Carrera, and Juan Carlos Laguna
May 2009, Neuroscience letters,
Laia Vilà, and Núria Roglans, and Marta Alegret, and Antoni Camins, and Mercè Pallàs, and Rosa María Sánchez, and Manuel Vázquez-Carrera, and Juan Carlos Laguna
December 1998, CNS drug reviews,
Laia Vilà, and Núria Roglans, and Marta Alegret, and Antoni Camins, and Mercè Pallàs, and Rosa María Sánchez, and Manuel Vázquez-Carrera, and Juan Carlos Laguna
February 2002, The journals of gerontology. Series A, Biological sciences and medical sciences,
Copied contents to your clipboard!