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After isoelectric focusing of proteins of human pure pancreatic juice following digestive enzymes and zymogens were identified by substrate specific detection methods: in the anodic range trypsinogen 1, and acidic form of proelastase and procarboxypeptidase A; in the pH--range of 5.0-6.0 a trypsinogen--like protein and the secretory trypsin inhibitor; in the pH--range of 6.0-7.0 one form of each of procarboxypeptidase B, trypsinogen 2, lipase, chymotrypsinogen and two forms of alpha-amylase and in the cathodic range an activated form of procarboxypeptidase B, one form of prophospholipase A2 and an additional form of proelastase. In order to characterize glycoproteins the IEF separated pancreatic proteins were transferred onto nitrocellulose membranes. Using the periodic acid/Schiff's reagent (PAS) procedure five PAS-positive proteins (pl s 4.5; 6.0; 6.6; 6.9 and 7.3) were detected. Three Con A-binding proteins (pl s 6.0; 6.6 and 6.9) were identified by incubating the blot in Con A followed by peroxidase and o-phenylendiamin as a chromogenic peroxidase substrat.
M Hänsler, and
G Appelt, and
R Rogos
October 1951,
Gastroenterology,
M Hänsler, and
G Appelt, and
R Rogos
March 1975,
Archives francaises des maladies de l'appareil digestif,
M Hänsler, and
G Appelt, and
R Rogos
December 1980,
Journal of chromatography,
M Hänsler, and
G Appelt, and
R Rogos
January 1976,
Virology,
M Hänsler, and
G Appelt, and
R Rogos
January 1987,
Deutsche Zeitschrift fur Verdauungs- und Stoffwechselkrankheiten,
M Hänsler, and
G Appelt, and
R Rogos
April 1977,
The Journal of parasitology,
M Hänsler, and
G Appelt, and
R Rogos
January 2003,
Methods in molecular biology (Clifton, N.J.),
M Hänsler, and
G Appelt, and
R Rogos
May 1998,
Electrophoresis,
M Hänsler, and
G Appelt, and
R Rogos
March 1998,
Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao,
M Hänsler, and
G Appelt, and
R Rogos
October 1971,
Nordisk medicin,
