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In this study, a preparative chromatography method named preparative argentation reversed-phase high performance liquid chromatography (Ag-RP-HPLC) was developed by adding silver ion to the mobile phase of preparative HPLC. Firstly, an analytical Ag-RP-HPLC method was developed, and the effects of silver content and acid content in the mobile phase on the column efficiency were studied. Based on the method of linear amplification, a preparative Ag-RP-HPLC technique with optimized separation conditions was developed. The new technique was applied successfully to the separation of the unsaturated aliphatic acid amide isomers contained in Asarum forbesii Maxim. Compared with the commonly used technique of argentation normal phase chromatography, this method with little solvent consumption is simple, fast, efficient, and flexible for the isolation and purification of the unsaturated compounds.
Feng Zhang, and
Xiaogang Chu, and
Xiujuan Wang, and
Minli Yang, and
Yun Ling, and
Li Sun, and
Xiuqin Li, and
Wei Yong
June 1987,
Journal of chromatography,
Feng Zhang, and
Xiaogang Chu, and
Xiujuan Wang, and
Minli Yang, and
Yun Ling, and
Li Sun, and
Xiuqin Li, and
Wei Yong
May 2001,
Se pu = Chinese journal of chromatography,
Feng Zhang, and
Xiaogang Chu, and
Xiujuan Wang, and
Minli Yang, and
Yun Ling, and
Li Sun, and
Xiuqin Li, and
Wei Yong
April 1984,
Journal of chromatography,
Feng Zhang, and
Xiaogang Chu, and
Xiujuan Wang, and
Minli Yang, and
Yun Ling, and
Li Sun, and
Xiuqin Li, and
Wei Yong
January 2004,
Methods in molecular biology (Clifton, N.J.),
Feng Zhang, and
Xiaogang Chu, and
Xiujuan Wang, and
Minli Yang, and
Yun Ling, and
Li Sun, and
Xiuqin Li, and
Wei Yong
January 1984,
Methods in enzymology,
Feng Zhang, and
Xiaogang Chu, and
Xiujuan Wang, and
Minli Yang, and
Yun Ling, and
Li Sun, and
Xiuqin Li, and
Wei Yong
May 2001,
Se pu = Chinese journal of chromatography,
Feng Zhang, and
Xiaogang Chu, and
Xiujuan Wang, and
Minli Yang, and
Yun Ling, and
Li Sun, and
Xiuqin Li, and
Wei Yong
January 1988,
Bioorganicheskaia khimiia,
Feng Zhang, and
Xiaogang Chu, and
Xiujuan Wang, and
Minli Yang, and
Yun Ling, and
Li Sun, and
Xiuqin Li, and
Wei Yong
April 2005,
Analytical and bioanalytical chemistry,
Feng Zhang, and
Xiaogang Chu, and
Xiujuan Wang, and
Minli Yang, and
Yun Ling, and
Li Sun, and
Xiuqin Li, and
Wei Yong
November 1986,
Journal of chromatography,
Feng Zhang, and
Xiaogang Chu, and
Xiujuan Wang, and
Minli Yang, and
Yun Ling, and
Li Sun, and
Xiuqin Li, and
Wei Yong
January 1987,
Journal of chromatography,
