BIOMAT Database Logo BIOMAT Database Logo

Comparison of oxine and tropolone methods for labeling human platelets with indium-111. 1991

H F Kotzé, and A D Heyns, and M G Lötter, and H Pieters, and J P Roodt, and M A Sweetlove, and P N Badenhorst
MRC Blood Platelet Research Unit, University of the Orange Free State, Bloemfontein, South Africa.

The effect of the chelates oxine and tropolone, used to label platelets, on the kinetics of indium-111-(111In) labeled platelets was studied in twelve normal human subjects. Autologous platelets were labeled either in saline with 111In-oxine or in plasma with 111In-tropolone. Mean platelet lifespan was estimated by fitting the disappearance curve of platelets from the circulation to the multiple hit and other mathematical models. The in vivo distribution of platelets was quantitatively imaged with a scintillation camera. The in vivo recovery of 111In-oxine and 111In-tropolone did not differ, and the mean platelet lifespan was also similar (111In-oxine: 230 +/- 29 hr; 111In-tropolone: 226 +/- 13 hr). At equilibrium (90 min after reinjection of labeled platelets) and at the end of platelet lifespan, 111In-oxine and 111In-tropolone radioactivities in the spleen and liver were similar. These results demonstrate that the results of kinetics measured with 111In-oxine or 111In-tropolone do not differ significantly.

UI MeSH Term Description Entries
D007205 Indium Radioisotopes Unstable isotopes of indium that decay or disintegrate emitting radiation. In atoms with atomic weights 106-112, 113m, 114, and 116-124 are radioactive indium isotopes. Radioisotopes, Indium
D007553 Isotope Labeling Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms. Isotope Labeling, Stable,Isotope-Coded Affinity Tagging,Isotopically-Coded Affinity Tagging,Affinity Tagging, Isotope-Coded,Affinity Tagging, Isotopically-Coded,Isotope Coded Affinity Tagging,Labeling, Isotope,Labeling, Stable Isotope,Stable Isotope Labeling,Tagging, Isotope-Coded Affinity,Tagging, Isotopically-Coded Affinity
D008297 Male Males
D009942 Organometallic Compounds A class of compounds of the type R-M, where a C atom is joined directly to any other element except H, C, N, O, F, Cl, Br, I, or At. (Grant & Hackh's Chemical Dictionary, 5th ed) Metallo-Organic Compound,Metallo-Organic Compounds,Metalloorganic Compound,Organometallic Compound,Metalloorganic Compounds,Compound, Metallo-Organic,Compound, Metalloorganic,Compound, Organometallic,Compounds, Metallo-Organic,Compounds, Metalloorganic,Compounds, Organometallic,Metallo Organic Compound,Metallo Organic Compounds
D001792 Blood Platelets Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation. Platelets,Thrombocytes,Blood Platelet,Platelet,Platelet, Blood,Platelets, Blood,Thrombocyte
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D014334 Tropolone A seven-membered aromatic ring compound. It is structurally related to a number of naturally occurring antifungal compounds (ANTIFUNGAL AGENTS).

Related Publications

H F Kotzé, and A D Heyns, and M G Lötter, and H Pieters, and J P Roodt, and M A Sweetlove, and P N Badenhorst
Neutrophil labeling with indium-111: tropolone vs. oxine.
November 1983, Radiology,
H F Kotzé, and A D Heyns, and M G Lötter, and H Pieters, and J P Roodt, and M A Sweetlove, and P N Badenhorst
Indium-111 tropolone versus oxine.
April 1983, Journal of nuclear medicine : official publication, Society of Nuclear Medicine,
H F Kotzé, and A D Heyns, and M G Lötter, and H Pieters, and J P Roodt, and M A Sweetlove, and P N Badenhorst
Re: indium-111 tropolone versus oxine.
May 1982, Journal of nuclear medicine : official publication, Society of Nuclear Medicine,
H F Kotzé, and A D Heyns, and M G Lötter, and H Pieters, and J P Roodt, and M A Sweetlove, and P N Badenhorst
Indium-111 platelet kinetics in normal human subjects: tropolone versus oxine methods.
November 1986, Journal of nuclear medicine : official publication, Society of Nuclear Medicine,
H F Kotzé, and A D Heyns, and M G Lötter, and H Pieters, and J P Roodt, and M A Sweetlove, and P N Badenhorst
Simple method for labeling of porcine platelets with indium-111 oxine.
April 1995, Laboratory animal science,
H F Kotzé, and A D Heyns, and M G Lötter, and H Pieters, and J P Roodt, and M A Sweetlove, and P N Badenhorst
[The Royal Spanish Pharmacopoeia: labeling platelets with (111)indium-oxine].
June 2000, Revista espanola de medicina nuclear,
H F Kotzé, and A D Heyns, and M G Lötter, and H Pieters, and J P Roodt, and M A Sweetlove, and P N Badenhorst
Labeling autologous leukocytes with indium-111 oxine.
June 1980, American journal of hospital pharmacy,
H F Kotzé, and A D Heyns, and M G Lötter, and H Pieters, and J P Roodt, and M A Sweetlove, and P N Badenhorst
A modified method for labeling human platelets with indium-111 oxine using albumin density-gradient separation.
October 1982, Radiology,
H F Kotzé, and A D Heyns, and M G Lötter, and H Pieters, and J P Roodt, and M A Sweetlove, and P N Badenhorst
Use of indium-111/oxine to label human platelets.
August 1978, Lancet (London, England),
H F Kotzé, and A D Heyns, and M G Lötter, and H Pieters, and J P Roodt, and M A Sweetlove, and P N Badenhorst
[Factors influencing platelets labelling with indium-111 oxine].
August 2021, Annales de biologie clinique,
Copied contents to your clipboard!