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Two substances, cAMP and 2,3-dimercapto-1-propanol (BAL) are known to induce transient activation of adenylate cyclase in Dictyostelium discoideum. A frigid mutant (HC85) has a deletion in a gene for G alpha 2, a guanine nucleotide binding protein and cannot activate the cyclase in response to cAMP. We found that BAL induced activation in the frigid mutant. This result suggests that the BAL-induced activation is independent of G alpha 2 and that BAL mimics a role of activated G alpha 2. We also found that cAMP promoted the BAL-induced activation. This result suggests that cAMP plays a role in activation through a mechanism in which G alpha 2 is not involved. We lastly showed that continuous cAMP stimulation could not inhibit the BAL-induced activation in the frigid mutant. Since the cAMP-induced inhibition observed in the wild type strain (NC4) proceeds with the time course identical to the cAMP-induced adaptation (Oyama, submitted), this result suggests that G alpha 2 is involved in adaptation of adenylate cyclase.
M Oyama, and
K Kubota, and
K Okamoto
January 1985,
Journal of cyclic nucleotide and protein phosphorylation research,
M Oyama, and
K Kubota, and
K Okamoto
January 1987,
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M Oyama, and
K Kubota, and
K Okamoto
October 1981,
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M Oyama, and
K Kubota, and
K Okamoto
January 1990,
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M Oyama, and
K Kubota, and
K Okamoto
March 1992,
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M Oyama, and
K Kubota, and
K Okamoto
October 1988,
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M Oyama, and
K Kubota, and
K Okamoto
January 1982,
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M Oyama, and
K Kubota, and
K Okamoto
February 1987,
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M Oyama, and
K Kubota, and
K Okamoto
March 1985,
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M Oyama, and
K Kubota, and
K Okamoto
October 1976,
FEBS letters,
