Biomaterial Database

Prevalence and persistence of polyagglutinable Pseudomonas aeruginosa in isolates from cystic fibrosis patients. 1991

B Ojeniyi, and N Høiby, and V T Rosdahl

Statens Seruminstitut, Copenhagen, Denmark.

During the 17 years from 1972 to 1988 1010 Pseudomonas aeruginosa isolates from sputum samples of 183 cystic fibrosis patients attending the Danish Cystic Fibrosis Centre at Rigshospitalet were serogrouped and phage typed. The patients were chronically infected with Pseudomonas aeruginosa (range of duration of infection: 1-17 years). They attended the Centre monthly and since 1976 all had been hospitalized for 14 days every three months. The number of patients with Pseudomonas aeruginosa infection increased from 29 to 123 during the period 1972 to 1988 and the number of isolates typed ranged from 14 to 197 a year. About half of the patients had one to five monoagglutinable isolates before they had polyagglutinable isolates. The prevalence of polyagglutinable isolates each year increased during the observation period (range: 24% to 88%). In all, three quarters of the patients had polyagglutinable isolates in their last typed sample. During the 17-year period 81% of the patients harboured polyagglutinable isolates. 77% of these patients, however, were for periods colonized with monoagglutinable isolates (0-3, 0-6, 0-1, 0-2 (including 0-2, 0-5, 0-2/5 due to cross-reactions between 0-2 and 0-5) and 0-9 were most prevalent) either alone or concurrent with polyagglutinable isolates, whereas 5% harboured polyagglutinable isolates exclusively during the whole observed period of infection. Polyagglutinable isolates with a short phage pattern were more frequent than those with a long phage pattern during the entire period from 1972 to 1988, and the prevalence increased during the period. Twenty-eight percent of the patients had a persistent phage pattern for an average of seven years in spite of change of serogroup. Twenty-seven percent of the patients had a persistent monoagglutinable serogroup and phage pattern for an average of eight years. The increasing prevalence and persistence of polyagglutinable strains correlate with the improved survival of the patients and thereby with the increased duration of the infection. The reason for this is discussed.

UI	MeSH Term	Description	Entries
D011550	Pseudomonas aeruginosa	A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.	Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D002908	Chronic Disease	Diseases which have one or more of the following characteristics: they are permanent, leave residual disability, are caused by nonreversible pathological alteration, require special training of the patient for rehabilitation, or may be expected to require a long period of supervision, observation, or care (Dictionary of Health Services Management, 2d ed). For epidemiological studies chronic disease often includes HEART DISEASES; STROKE; CANCER; and diabetes (DIABETES MELLITUS, TYPE 2).	Chronic Condition,Chronic Illness,Chronically Ill,Chronic Conditions,Chronic Diseases,Chronic Illnesses,Condition, Chronic,Disease, Chronic,Illness, Chronic
D003550	Cystic Fibrosis	An autosomal recessive genetic disease of the EXOCRINE GLANDS. It is caused by mutations in the gene encoding the CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR expressed in several organs including the LUNG, the PANCREAS, the BILIARY SYSTEM, and the SWEAT GLANDS. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in AIRWAY OBSTRUCTION; chronic RESPIRATORY INFECTIONS; PANCREATIC INSUFFICIENCY; maldigestion; salt depletion; and HEAT PROSTRATION.	Mucoviscidosis,Cystic Fibrosis of Pancreas,Fibrocystic Disease of Pancreas,Pancreatic Cystic Fibrosis,Pulmonary Cystic Fibrosis,Cystic Fibrosis, Pancreatic,Cystic Fibrosis, Pulmonary,Fibrosis, Cystic,Pancreas Fibrocystic Disease,Pancreas Fibrocystic Diseases
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000371	Agglutination	The clumping together of suspended material resulting from the action of AGGLUTININS.
D001434	Bacteriophage Typing	A technique of bacterial typing which differentiates between bacteria or strains of bacteria by their susceptibility to one or more bacteriophages.	Phage Typing,Typing, Bacteriophage,Typing, Phage
D012703	Serotyping	Process of determining and distinguishing species of bacteria or viruses based on antigens they share.	Serotypings