-transparent.png){kind=logo}
A gene encoding a variant (lacking amino acids 6-173) of human tissue-type plasminogen activator (t-PA), consisting only of the second kringle domain (K2) and the serine protease domain (P), was fused to a DNA segment coding for the signal peptide of staphylococcal protein A and a synthetic gene coding for a protein with ability to bind immunoglobulin G (IgG). The fusion protein which was synthesized in Escherichia coli and secreted into the growth medium, was found to be fibrinolytically active. Purification of the fusion protein was performed in a single step by affinity chromatography with immobilized IgG. Enzymatically active K2P was liberated from the fusion protein by cleavage at a unique Asn-Gly dipeptide sequence using hydroxylamine. These results demonstrate that a variant of human t-PA can be synthesized and secreted by E. coli as a fibrinolytically active fusion protein, which upon specific cleavage yields an active variant t-PA of the expected size.
M Waldenström, and
E Holmgren, and
A Attersand, and
C Kalderén, and
B Löwenadler, and
B Rådén, and
L Hansson, and
G Pohl
June 2001,
Applied and environmental microbiology,
M Waldenström, and
E Holmgren, and
A Attersand, and
C Kalderén, and
B Löwenadler, and
B Rådén, and
L Hansson, and
G Pohl
December 1998,
Applied and environmental microbiology,
M Waldenström, and
E Holmgren, and
A Attersand, and
C Kalderén, and
B Löwenadler, and
B Rådén, and
L Hansson, and
G Pohl
March 2007,
Pakistan journal of biological sciences : PJBS,
M Waldenström, and
E Holmgren, and
A Attersand, and
C Kalderén, and
B Löwenadler, and
B Rådén, and
L Hansson, and
G Pohl
August 2001,
Seminars in thrombosis and hemostasis,
M Waldenström, and
E Holmgren, and
A Attersand, and
C Kalderén, and
B Löwenadler, and
B Rådén, and
L Hansson, and
G Pohl
December 2005,
Applied and environmental microbiology,
M Waldenström, and
E Holmgren, and
A Attersand, and
C Kalderén, and
B Löwenadler, and
B Rådén, and
L Hansson, and
G Pohl
January 1991,
The Journal of biological chemistry,
M Waldenström, and
E Holmgren, and
A Attersand, and
C Kalderén, and
B Löwenadler, and
B Rådén, and
L Hansson, and
G Pohl
January 1994,
Biotechnology progress,
M Waldenström, and
E Holmgren, and
A Attersand, and
C Kalderén, and
B Löwenadler, and
B Rådén, and
L Hansson, and
G Pohl
December 1990,
Gene,
M Waldenström, and
E Holmgren, and
A Attersand, and
C Kalderén, and
B Löwenadler, and
B Rådén, and
L Hansson, and
G Pohl
June 2011,
Pharmaceutical biology,
M Waldenström, and
E Holmgren, and
A Attersand, and
C Kalderén, and
B Löwenadler, and
B Rådén, and
L Hansson, and
G Pohl
January 2002,
Arzneimittel-Forschung,
