Proteomic analysis of homocysteine induced proliferation of cultured neonatal rat vascular smooth muscle cells. 2009

Xiaohua Liu, and Jing Shen, and Rui Zhan, and Xingxing Wang, and Xiaoming Wang, and Zhiqing Zhang, and Xue Leng, and Zhihua Yang, and Lingjia Qian
Department of Stress Medicine, Institute of Health and Environmental Medicine, Tianjin 300050, People's Republic of China.

Hyperhomocysteinemia is a common independent risk factor for cardiovascular diseases. The promoting effect of homocysteine (Hcy) on vascular smooth muscle cell (VSMC) proliferation has been considered as one of the important pathological bases of atherosclerosis. However, the mechanism of VSMC proliferation induced by Hcy remains unclear. The present research used proteomic techniques to globally analyze the protein changes in proliferative VSMCs. After comparing the protein expression profiles of VSMCs between the Hcy-treated and non-treated groups, 11 protein spots were found altered markedly in proliferative VSMCs with expression of eight protein spots increased and three protein spots decreased. In the differentially expressed proteins, eight protein spots were identified successfully including glycolytic metabolism proteins: pyruvate kinase M2 (PKM2), triosephosphate isomerase (TPI) and aldose reductase (AR); cytoskeletal proteins: lamin C and vimentin; and three other proteins: calreticulin; similar to WDR1 protein and LIM and SH3 protein 1. The differentially expressed proteins were further validated by Western blot and confirmed by assay of enzymes' activities and ATP content. These results may provide some clues for comprehensively understanding the mechanism of VSMC proliferation and pathogenesis of atherosclerosis induced by Hcy.

UI MeSH Term Description Entries
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D006710 Homocysteine A thiol-containing amino acid formed by a demethylation of METHIONINE. 2-amino-4-mercaptobutyric acid,Homocysteine, L-Isomer,2 amino 4 mercaptobutyric acid,Homocysteine, L Isomer,L-Isomer Homocysteine
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000831 Animals, Newborn Refers to animals in the period of time just after birth. Animals, Neonatal,Animal, Neonatal,Animal, Newborn,Neonatal Animal,Neonatal Animals,Newborn Animal,Newborn Animals
D001013 Aorta, Thoracic The portion of the descending aorta proceeding from the arch of the aorta and extending to the DIAPHRAGM, eventually connecting to the ABDOMINAL AORTA. Aorta, Ascending,Aorta, Descending,Aortic Arch,Aortic Root,Arch of the Aorta,Descending Aorta,Sinotubular Junction,Ascending Aorta,Thoracic Aorta,Aortic Roots,Arch, Aortic,Ascending Aortas,Junction, Sinotubular,Root, Aortic,Sinotubular Junctions
D015180 Electrophoresis, Gel, Two-Dimensional Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels. Gel Electrophoresis, Two-Dimensional,Polyacrylamide Gel Electrophoresis, Two-Dimensional,2-D Gel Electrophoresis,2-D Polyacrylamide Gel Electrophoresis,2D Gel Electrophoresis,2D PAGE,2D Polyacrylamide Gel Electrophoresis,Electrophoresis, Gel, 2-D,Electrophoresis, Gel, 2D,Electrophoresis, Gel, Two Dimensional,Polyacrylamide Gel Electrophoresis, 2-D,Polyacrylamide Gel Electrophoresis, 2D,Two Dimensional Gel Electrophoresis,2 D Gel Electrophoresis,2 D Polyacrylamide Gel Electrophoresis,Electrophoresis, 2-D Gel,Electrophoresis, 2D Gel,Electrophoresis, Two-Dimensional Gel,Gel Electrophoresis, 2-D,Gel Electrophoresis, 2D,Gel Electrophoresis, Two Dimensional,PAGE, 2D,Polyacrylamide Gel Electrophoresis, 2 D,Polyacrylamide Gel Electrophoresis, Two Dimensional,Two-Dimensional Gel Electrophoresis
D017208 Rats, Wistar A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain. Wistar Rat,Rat, Wistar,Wistar Rats
D049109 Cell Proliferation All of the processes involved in increasing CELL NUMBER including CELL DIVISION. Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D019032 Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis. Laser Desorption-Ionization Mass Spectrometry, Matrix-Assisted,MALD-MS,MALDI,Mass Spectrometry, Matrix-Assisted Laser Desorption-Ionization,Mass Spectroscopy, Matrix-Assisted Laser Desorption-Ionization,Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry,Spectroscopy, Mass, Matrix-Assisted Laser Desorption-Ionization,MALDI-MS,MS-MALD,SELDI-TOF-MS,Surface Enhanced Laser Desorption Ionization Mass Spectrometry,Laser Desorption Ionization Mass Spectrometry, Matrix Assisted,MALDI MS,Mass Spectrometry, Matrix Assisted Laser Desorption Ionization,Mass Spectroscopy, Matrix Assisted Laser Desorption Ionization,Matrix Assisted Laser Desorption Ionization Mass Spectrometry

Related Publications

Xiaohua Liu, and Jing Shen, and Rui Zhan, and Xingxing Wang, and Xiaoming Wang, and Zhiqing Zhang, and Xue Leng, and Zhihua Yang, and Lingjia Qian
June 1999, Zhonghua yi xue za zhi,
Xiaohua Liu, and Jing Shen, and Rui Zhan, and Xingxing Wang, and Xiaoming Wang, and Zhiqing Zhang, and Xue Leng, and Zhihua Yang, and Lingjia Qian
May 2004, Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences,
Xiaohua Liu, and Jing Shen, and Rui Zhan, and Xingxing Wang, and Xiaoming Wang, and Zhiqing Zhang, and Xue Leng, and Zhihua Yang, and Lingjia Qian
December 2003, Life sciences,
Xiaohua Liu, and Jing Shen, and Rui Zhan, and Xingxing Wang, and Xiaoming Wang, and Zhiqing Zhang, and Xue Leng, and Zhihua Yang, and Lingjia Qian
June 2005, The Indian journal of medical research,
Xiaohua Liu, and Jing Shen, and Rui Zhan, and Xingxing Wang, and Xiaoming Wang, and Zhiqing Zhang, and Xue Leng, and Zhihua Yang, and Lingjia Qian
July 1994, The American journal of physiology,
Xiaohua Liu, and Jing Shen, and Rui Zhan, and Xingxing Wang, and Xiaoming Wang, and Zhiqing Zhang, and Xue Leng, and Zhihua Yang, and Lingjia Qian
December 2010, European journal of pharmacology,
Xiaohua Liu, and Jing Shen, and Rui Zhan, and Xingxing Wang, and Xiaoming Wang, and Zhiqing Zhang, and Xue Leng, and Zhihua Yang, and Lingjia Qian
December 2011, Journal of cardiovascular pharmacology,
Xiaohua Liu, and Jing Shen, and Rui Zhan, and Xingxing Wang, and Xiaoming Wang, and Zhiqing Zhang, and Xue Leng, and Zhihua Yang, and Lingjia Qian
February 2018, Molecular and cellular biochemistry,
Xiaohua Liu, and Jing Shen, and Rui Zhan, and Xingxing Wang, and Xiaoming Wang, and Zhiqing Zhang, and Xue Leng, and Zhihua Yang, and Lingjia Qian
June 2007, Clinical nutrition (Edinburgh, Scotland),
Xiaohua Liu, and Jing Shen, and Rui Zhan, and Xingxing Wang, and Xiaoming Wang, and Zhiqing Zhang, and Xue Leng, and Zhihua Yang, and Lingjia Qian
March 2007, The Canadian journal of cardiology,
Copied contents to your clipboard!