Spatiotemporal expression of Hsp20 and its phosphorylation in hippocampal CA1 pyramidal neurons after transient forebrain ischemia. 2009

Masayuki Niwa, and Akira Hara, and Ayako Taguchi, and Hitomi Aoki, and Osamu Kozawa, and Hideki Mori
Medical Science Division, United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu, Japan; Medical Education Development Center, Graduate School of Medicine, Gifu University, Gifu, Japan. mniwa@gifu-u.ac.jp

The goal of this study was to analyze the spatiotemporal expression of heat shock protein 20 (Hsp20) and its phosphorylation in gerbil brain after transient forebrain ischemia. Brain sections from Mongolian gerbil killed 24, 48, 72 and 96 hours and 2 weeks after ischemia (n=5 in each experimental group) were evaluated with immunohistochemical and in situ DNA end-labeling [terminal 2'-deoxyuridine 5'-triphosphate nick end-labeling (TUNEL)] techniques. Ischemia-associated Hsp20 expression was observed 24 and 48 hours later in the area of the stratum radiatum and then disappeared by 72 hours. This staining appeared along the lines of apical dendrites. Hsp20 staining in the stratum pyramidale was observed again 2 weeks after ischemia. Strong immunoreactivity for phosphorylation markers was observed in the stratum pyramidale 2 weeks after ischemia, whereas no staining was seen at either 24 or 48 hours after ischemia. Fragmented DNA was observed in nuclei and apical dendrites of CA1 pyramidal neurons by TUNEL method between 72 and 96 hours after reperfusion. The emerging expression of the Hsp20 protein within the restricted location of CA1 before the fragmented DNA transport suggests the strong relationship between Hsp20 and CA1 neuronal cell apoptosis. These findings imply a two-phase role of Hsp20 in brain ischemia: an acute phase before DNA fragmentation and a subacute phase 2 weeks after ischemia. The former may be associated with apoptosis with fragmented nuclear DNA transport into neuronal fibers and the latter associated with glial response to ischemic insult. Phosphorylation of Hsp20 might contribute to the subacute phase but not to an acute phase.

UI MeSH Term Description Entries
D008297 Male Males
D010766 Phosphorylation The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety. Phosphorylations
D002546 Ischemic Attack, Transient Brief reversible episodes of focal, nonconvulsive ischemic dysfunction of the brain having a duration of less than 24 hours, and usually less than one hour, caused by transient thrombotic or embolic blood vessel occlusion or stenosis. Events may be classified by arterial distribution, temporal pattern, or etiology (e.g., embolic vs. thrombotic). (From Adams et al., Principles of Neurology, 6th ed, pp814-6) Brain Stem Ischemia, Transient,Cerebral Ischemia, Transient,Crescendo Transient Ischemic Attacks,Transient Ischemic Attack,Anterior Circulation Transient Ischemic Attack,Brain Stem Transient Ischemic Attack,Brain TIA,Brainstem Ischemia, Transient,Brainstem Transient Ischemic Attack,Carotid Circulation Transient Ischemic Attack,Posterior Circulation Transient Ischemic Attack,TIA (Transient Ischemic Attack),Transient Ischemic Attack, Anterior Circulation,Transient Ischemic Attack, Brain Stem,Transient Ischemic Attack, Brainstem,Transient Ischemic Attack, Carotid Circulation,Transient Ischemic Attack, Posterior Circulation,Transient Ischemic Attack, Vertebrobasilar Circulation,Transient Ischemic Attacks, Crescendo,Vertebrobasilar Circulation Transient Ischemic Attack,Attack, Transient Ischemic,Attacks, Transient Ischemic,Brainstem Ischemias, Transient,Cerebral Ischemias, Transient,Ischemia, Transient Brainstem,Ischemia, Transient Cerebral,Ischemias, Transient Brainstem,Ischemias, Transient Cerebral,Ischemic Attacks, Transient,TIA, Brain,TIAs (Transient Ischemic Attack),Transient Brainstem Ischemia,Transient Cerebral Ischemia,Transient Cerebral Ischemias,Transient Ischemic Attacks
D004195 Disease Models, Animal Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases. Animal Disease Model,Animal Disease Models,Disease Model, Animal
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D005849 Gerbillinae A subfamily of the Muridae consisting of several genera including Gerbillus, Rhombomys, Tatera, Meriones, and Psammomys. Gerbils,Jird,Meriones,Psammomys,Rats, Sand,Gerbil,Jirds,Merione,Rat, Sand,Sand Rat,Sand Rats
D005904 Glial Fibrillary Acidic Protein An intermediate filament protein found only in glial cells or cells of glial origin. MW 51,000. Glial Intermediate Filament Protein,Astroprotein,GFA-Protein,Glial Fibrillary Acid Protein,GFA Protein
D006624 Hippocampus A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation. Ammon Horn,Cornu Ammonis,Hippocampal Formation,Subiculum,Ammon's Horn,Hippocampus Proper,Ammons Horn,Formation, Hippocampal,Formations, Hippocampal,Hippocampal Formations,Hippocampus Propers,Horn, Ammon,Horn, Ammon's,Proper, Hippocampus,Propers, Hippocampus,Subiculums
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor

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