BIOMAT Database Logo BIOMAT Database Logo

Caspase-independent cell death without generation of reactive oxygen species in irradiated MOLT-4 human leukemia cells. 2009

Kengo Yoshida, and Yoshiko Kubo, and Yoichiro Kusunoki, and Yukari Morishita, and Hiroko Nagamura, and Ikue Hayashi, and Seishi Kyoizumi, and Toshio Seyama, and Kei Nakachi, and Tomonori Hayashi
Department of Radiobiology/Molecular Epidemiology, Radiation Effects Research Foundation, 5-2 Hijiyama Park, Minami-ku, Hiroshima 732-0815, Japan.

To improve our understanding of ionizing radiation effects on immune cells, we investigated steps leading to radiation-induced cell death in MOLT-4, a thymus-derived human leukemia cell. After exposure of MOLT-4 cells to 4 Gy of X-rays, irradiated cells sequentially showed increase in intracellular reactive oxygen species (ROS), decrease in mitochondrial membrane potential, and eventually apoptotic cell death. In the presence of the caspase inhibitor z-VAD-fmk, irradiated cells exhibited necrotic characteristics such as mitochondrial swelling instead of apoptosis. ROS generation was not detected during this necrotic cell death process. These results indicate that radiation-induced apoptosis in MOLT-4 cells requires elevation of intracellular ROS as well as activation of a series of caspases, whereas the cryptic necrosis program--which is independent of intracellular ROS generation and caspase activation--is activated when the apoptosis pathway is blocked.

UI MeSH Term Description Entries
D007938 Leukemia A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006) Leucocythaemia,Leucocythemia,Leucocythaemias,Leucocythemias,Leukemias
D008928 Mitochondria Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed) Mitochondrial Contraction,Mitochondrion,Contraction, Mitochondrial,Contractions, Mitochondrial,Mitochondrial Contractions
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000590 Amino Acid Chloromethyl Ketones Inhibitors of SERINE ENDOPEPTIDASES and sulfhydryl group-containing enzymes. They act as alkylating agents and are known to interfere in the translation process. Peptide Chloromethyl Ketones,Chloromethyl Ketones, Peptide,Ketones, Peptide Chloromethyl
D014965 X-Rays Penetrating electromagnetic radiation emitted when the inner orbital electrons of an atom are excited and release radiant energy. X-ray wavelengths range from 1 pm to 10 nm. Hard X-rays are the higher energy, shorter wavelength X-rays. Soft x-rays or Grenz rays are less energetic and longer in wavelength. The short wavelength end of the X-ray spectrum overlaps the GAMMA RAYS wavelength range. The distinction between gamma rays and X-rays is based on their radiation source. Grenz Ray,Grenz Rays,Roentgen Ray,Roentgen Rays,X Ray,X-Ray,Xray,Radiation, X,X-Radiation,Xrays,Ray, Grenz,Ray, Roentgen,Ray, X,Rays, Grenz,Rays, Roentgen,Rays, X,X Radiation,X Rays,X-Radiations
D016923 Cell Death The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability. Death, Cell
D017382 Reactive Oxygen Species Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of SIGNAL TRANSDUCTION and GENE EXPRESSION, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS. Active Oxygen Species,Oxygen Radical,Oxygen Radicals,Pro-Oxidant,Reactive Oxygen Intermediates,Active Oxygen,Oxygen Species, Reactive,Pro-Oxidants,Oxygen, Active,Pro Oxidant,Pro Oxidants,Radical, Oxygen
D045304 Cytochromes c Cytochromes of the c type that are found in eukaryotic MITOCHONDRIA. They serve as redox intermediates that accept electrons from MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX III and transfer them to MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX IV. Cytochrome c,Ferricytochrome c,Ferrocytochrome c,Apocytochrome C
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D048430 Cell Shape The quality of surface form or outline of CELLS. Cell Shapes,Shape, Cell,Shapes, Cell

Related Publications

Kengo Yoshida, and Yoshiko Kubo, and Yoichiro Kusunoki, and Yukari Morishita, and Hiroko Nagamura, and Ikue Hayashi, and Seishi Kyoizumi, and Toshio Seyama, and Kei Nakachi, and Tomonori Hayashi
Melatonin induces apoptosis through a caspase-dependent but reactive oxygen species-independent mechanism in human leukemia Molt-3 cells.
September 2013, Journal of pineal research,
Kengo Yoshida, and Yoshiko Kubo, and Yoichiro Kusunoki, and Yukari Morishita, and Hiroko Nagamura, and Ikue Hayashi, and Seishi Kyoizumi, and Toshio Seyama, and Kei Nakachi, and Tomonori Hayashi
Time sequence analysis of caspase-3-independent programmed cell death and apoptosis in X-irradiated human leukemic MOLT-4 cells.
December 2002, Cell and tissue research,
Kengo Yoshida, and Yoshiko Kubo, and Yoichiro Kusunoki, and Yukari Morishita, and Hiroko Nagamura, and Ikue Hayashi, and Seishi Kyoizumi, and Toshio Seyama, and Kei Nakachi, and Tomonori Hayashi
Cytolethal distending toxin induces caspase-dependent and -independent cell death in MOLT-4 cells.
October 2008, Infection and immunity,
Kengo Yoshida, and Yoshiko Kubo, and Yoichiro Kusunoki, and Yukari Morishita, and Hiroko Nagamura, and Ikue Hayashi, and Seishi Kyoizumi, and Toshio Seyama, and Kei Nakachi, and Tomonori Hayashi
N-(4-Hydroxyphenyl)retinamide (4-HPR) induces leukemia cell death via generation of reactive oxygen species.
October 2003, International journal of hematology,
Kengo Yoshida, and Yoshiko Kubo, and Yoichiro Kusunoki, and Yukari Morishita, and Hiroko Nagamura, and Ikue Hayashi, and Seishi Kyoizumi, and Toshio Seyama, and Kei Nakachi, and Tomonori Hayashi
Gardenin B-induced cell death in human leukemia cells involves multiple caspases but is independent of the generation of reactive oxygen species.
August 2016, Chemico-biological interactions,
Kengo Yoshida, and Yoshiko Kubo, and Yoichiro Kusunoki, and Yukari Morishita, and Hiroko Nagamura, and Ikue Hayashi, and Seishi Kyoizumi, and Toshio Seyama, and Kei Nakachi, and Tomonori Hayashi
Reactive oxygen species are involved in FasL-induced caspase-independent cell death and inflammatory responses.
March 2009, Free radical biology & medicine,
Kengo Yoshida, and Yoshiko Kubo, and Yoichiro Kusunoki, and Yukari Morishita, and Hiroko Nagamura, and Ikue Hayashi, and Seishi Kyoizumi, and Toshio Seyama, and Kei Nakachi, and Tomonori Hayashi
Caspase inhibition sensitizes inhibitor of NF-kappaB kinase beta-deficient fibroblasts to caspase-independent cell death via the generation of reactive oxygen species.
June 2007, The Journal of biological chemistry,
Kengo Yoshida, and Yoshiko Kubo, and Yoichiro Kusunoki, and Yukari Morishita, and Hiroko Nagamura, and Ikue Hayashi, and Seishi Kyoizumi, and Toshio Seyama, and Kei Nakachi, and Tomonori Hayashi
Yeast cell death during DNA damage arrest is independent of caspase or reactive oxygen species.
August 2004, The Journal of cell biology,
Kengo Yoshida, and Yoshiko Kubo, and Yoichiro Kusunoki, and Yukari Morishita, and Hiroko Nagamura, and Ikue Hayashi, and Seishi Kyoizumi, and Toshio Seyama, and Kei Nakachi, and Tomonori Hayashi
IMMUNEPOTENT CRP induces cell cycle arrest and caspase-independent regulated cell death in HeLa cells through reactive oxygen species production.
January 2018, BMC cancer,
Kengo Yoshida, and Yoshiko Kubo, and Yoichiro Kusunoki, and Yukari Morishita, and Hiroko Nagamura, and Ikue Hayashi, and Seishi Kyoizumi, and Toshio Seyama, and Kei Nakachi, and Tomonori Hayashi
Benzene metabolites enhance reactive oxygen species generation in HL60 human leukemia cells.
May 1996, Human & experimental toxicology,
Copied contents to your clipboard!