Biomaterial Database

A kinetic study of irreversible enzyme inhibition by an inhibitor that is rendered unstable by enzymic catalysis. The inhibition of polyphenol oxidase by L-cysteine. 1991

E Valero, and R Varón, and F García-Carmona

Departamento de Química, Escuela Universitaria Politécnica de Albacete, Universidad de Castilla-La Mancha, Spain.

A kinetic study of the irreversible inhibition of an enzyme by an inhibitor that is depleted in the medium by its reaction with the product of enzymic analysis was made. The model is illustrated by the study of the inhibition of catecholase activity of polyphenol oxidase by L-cysteine. The inhibition is characterized by an initial lag period followed by a concomitant decrease in enzymic activity expressed when the steady state is reached, both kinetic parameters being modulated by enzyme, substrate and inhibitor concentrations. There is no analytical solution to the non-linear differential-equation system that describes the kinetics of the reaction, and so computer simulations of this dynamic behaviour are presented. The results obtained show that the system here studied presents kinetic co-operativity for a target enzyme that follows the simple Michaelis-Menten mechanism in its action on the substrate.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D010944	Plants	Multicellular, eukaryotic life forms of kingdom Plantae. Plants acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations. It is a non-taxonomical term most often referring to LAND PLANTS. In broad sense it includes RHODOPHYTA and GLAUCOPHYTA along with VIRIDIPLANTAE.	Plant
D003545	Cysteine	A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.	Cysteine Hydrochloride,Half-Cystine,L-Cysteine,Zinc Cysteinate,Half Cystine,L Cysteine
D004156	Catechol Oxidase	An enzyme of the oxidoreductase class that catalyzes the reaction between catechol and oxygen to yield benzoquinone and water. It is a complex of copper-containing proteins that acts also on a variety of substituted catechols. EC 1.10.3.1.	Diphenol Oxidases,Diphenol Oxidase,Polyphenol Oxidase,Polyphenoloxidase,Oxidase, Catechol,Oxidase, Diphenol,Oxidase, Polyphenol,Oxidases, Diphenol
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In