Biomaterial Database

Induction of leukemia inhibitory factor secretion by interleukin-1 in a human T lymphoma cell line. 1991

H Gascan, and J Lemetayer

INSERM U211, Nantes, France.

The control of leukemia inhibitory factor (LIF) secretion by interleukin-1 (IL-1) was studied in the HSB2 human T lymphoma cell line. We show that in the presence of phytohemagglutinin (PHA), the addition of IL-1 in the culture medium induced a strong increase in the level of secreted LIF, as detected with the DA1.a test cell line. Subclones of the HSB2 cell line obtained by limiting dilution, and selected for their high level of LIF secretion, after phorbol myristate acetate (PMA) treatment, were more carefully analyzed. In the same conditions of stimulation, a 50X increase in the amount of secreted LIF was observed with the 2B3 subclone, whereas the 3C2 subclone gave a very weak signal in response to the stimulation. Several other chemical reagents and a panel of recombinant cytokines including IL-2, -3, -4, -5, and -6, granulocyte monocyte colony-stimulating factor (GM-CSF), monocyte-CSF (M-CSF), tumor necrosis factor-alpha (TNF-alpha), and interferon-gamma (IFN-gamma) were tested for their induction of LIF secretion. None of the other tested components was as potent as IL-1 (100 U/ml) and 1% PHA. A half maximal response was obtained with a concentration of 15-20 pM IL-1.

UI	MeSH Term	Description	Entries
D007375	Interleukin-1	A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.	IL-1,Lymphocyte-Activating Factor,Epidermal Cell Derived Thymocyte-Activating Factor,Interleukin I,Macrophage Cell Factor,T Helper Factor,Epidermal Cell Derived Thymocyte Activating Factor,Interleukin 1,Lymphocyte Activating Factor
D008222	Lymphokines	Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.	Lymphocyte Mediators,Mediators, Lymphocyte
D006131	Growth Inhibitors	Endogenous or exogenous substances which inhibit the normal growth of human and animal cells or micro-organisms, as distinguished from those affecting plant growth (	Cell Growth Inhibitor,Cell Growth Inhibitors,Growth Inhibitor,Growth Inhibitor, Cell,Growth Inhibitors, Cell,Inhibitor, Cell Growth,Inhibitor, Growth,Inhibitors, Cell Growth,Inhibitors, Growth
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014407	Tumor Cells, Cultured	Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.	Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D015850	Interleukin-6	A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.	Hepatocyte-Stimulating Factor,Hybridoma Growth Factor,IL-6,MGI-2,Myeloid Differentiation-Inducing Protein,Plasmacytoma Growth Factor,B Cell Stimulatory Factor-2,B-Cell Differentiation Factor,B-Cell Differentiation Factor-2,B-Cell Stimulatory Factor 2,B-Cell Stimulatory Factor-2,BSF-2,Differentiation Factor, B-Cell,Differentiation Factor-2, B-Cell,IFN-beta 2,IL6,Interferon beta-2,B Cell Differentiation Factor,B Cell Differentiation Factor 2,B Cell Stimulatory Factor 2,Differentiation Factor 2, B Cell,Differentiation Factor, B Cell,Differentiation-Inducing Protein, Myeloid,Growth Factor, Hybridoma,Growth Factor, Plasmacytoma,Hepatocyte Stimulating Factor,Interferon beta 2,Interleukin 6,Myeloid Differentiation Inducing Protein,beta-2, Interferon
D016399	Lymphoma, T-Cell	A group of heterogeneous lymphoid tumors representing malignant transformations of T-lymphocytes.	T-Cell Lymphoma,Lymphoma, T Cell,Lymphomas, T-Cell,T Cell Lymphoma,T-Cell Lymphomas
D053605	Leukemia Inhibitory Factor	An INTERLEUKIN-6 related cytokine that exhibits pleiotrophic effects on many physiological systems that involve cell proliferation, differentiation, and survival. Leukemia inhibitory factor binds to and acts through the lif receptor.	AM424,Cholinergic Differentiation Factor,D Factor (Leukemia Inhibitory Factor),Differentiation-Stimulating Factor,Emfilermin,Myeloid Differentiation-Stimulating Factor,Differentiation Factor, Cholinergic,Differentiation Stimulating Factor,Differentiation-Stimulating Factor, Myeloid,Inhibitory Factor, Leukemia,Myeloid Differentiation Stimulating Factor