Biomaterial Database

[Preliminary study of naturally occurring anti-idiotypic antibodies in schistosomiasis japonica]. 1991

Y P Xing, and J W Hong, and G L Wu

Department of Parasitology, Nanjing Medical College.

In this study, naturally occurring anti-idiotypic antibodies in sera from patients with schistosomiasis japonica were demonstrated by using McAb (5B5H)-ELISA. McAb 5B5H against Schistosoma japonicum soluble egg antigen (SEA) was purified by precipitation with 50%, 40% and 33% saturated (NH4)2SO4 and coupled to CNBr-activated Sepharose 4B (Pharmacia). 5B5H column was loaded with sera from two patients with schistosomiasis japonica and then the bound material was eluated with 3M KCNS. The resulting eluate was further characterized with McAb-ELISA and complement fixation test in order to minimize the possible interference of circulating antigen-antibody complex. The results showed that the eluate contained mainly anti-Id antibodies. In the competitive 5B5H-ELISA tests, SEA markedly inhibited the interaction of 5B5H (Id) and anti-Id anti-bodies, suggesting that the relevant idiotype is within or adjacent closely to antigen binding site of anti-SEA molecule. The anti-Id antibodies were detected using 5B5H-ELISA in patients with schistosomiasis japonica from A and B areas, positive rate of 35.1% (13/37) and 60% (18/30) was obtained respectively. No cross or false reactions were found with sera from patients with fasciolopsiasis buski (0/15), clonorchiasis sinensis (0/15) and normal group of students (0/54) with the exception of one out of 20 patients with paragonimiasis westermani.

UI	MeSH Term	Description	Entries
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000888	Antibodies, Anti-Idiotypic	Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.	Anti-Antibodies,Anti-Idiotype Antibodies,Antibodies, Internal Image,Antigamma Globulin Antibodies,Antiglobulins,Anti Antibodies,Anti-gamma Globulin Antibodies,Anti Idiotype Antibodies,Anti gamma Globulin Antibodies,Anti-Idiotypic Antibodies,Antibodies, Anti,Antibodies, Anti Idiotypic,Antibodies, Anti-Idiotype,Antibodies, Anti-gamma Globulin,Antibodies, Antigamma Globulin,Globulin Antibodies, Anti-gamma,Globulin Antibodies, Antigamma,Image Antibodies, Internal,Internal Image Antibodies
D000909	Antibodies, Helminth	Immunoglobulins produced in a response to HELMINTH ANTIGENS.	Helminth Antibodies
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D012554	Schistosomiasis japonica	Schistosomiasis caused by Schistosoma japonicum. It is endemic in the ASIA, EASTERN and affects the bowel, liver, and spleen.	Schistosoma japonicum Infection,Schistosomiasis japonicum,Infection, Schistosoma japonicum,Infections, Schistosoma japonicum,Schistosoma japonicum Infections